TY - JOUR
T1 - Zn-or cu-containing cap-based coatings formed by micro-arc oxidation on titanium and ti-40nb alloy
T2 - Part ii—wettability and biological performance
AU - Komarova, Ekaterina G.
AU - Sharkeev, Yurii P.
AU - Sedelnikova, Mariya B.
AU - Prymak, Oleg
AU - Epple, Matthias
AU - Litvinova, Larisa S.
AU - Shupletsova, Valeria V.
AU - Malashchenko, Vladimir V.
AU - Yurova, Kristina A.
AU - Dzyuman, Anna N.
AU - Kulagina, Irina V.
AU - Mushtovatova, Lyudmila S.
AU - Bochkareva, Olga P.
AU - Karpova, Mariia R.
AU - Khlusov, Igor A.
N1 - Funding Information:
Funding: This research was funded by the Government research assignment for ISPMS SB RAS, Project No. III.23.2.5, and by the Russian Science Foundation, Grant No. 16-15-10031 (biological studies in vitro and in vivo of the CaP coatings on pure titanium).
Funding Information:
Acknowledgments: The authors wish to acknowledge assistance and encouragement from Marina V. Chaikina (Institute of Solid State Chemistry and Mechanochemistry SB RAS, Novosibirsk, Russia), and Valentina V. Chebodaeva (Institute of Strength Physics and Materials Science SB RAS, Tomsk, Russia). The authors also would like to acknowledge the opportunities provided by the German Academic Exchange Service (DAAD; Leonhard-Euler program) in terms of exchange programs and support of international collaboration, and by the framework of the Tomsk Polytechnic University Competitiveness Enhancement Program.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - This work describes the wettability and biological performance of Zn-and Cu-containing CaP-based coatings prepared by micro-arc oxidation on pure titanium (Ti) and novel Ti-40Nb alloy. Good hydrophilic properties of all the coatings were demonstrated by the low contact angles with liquids, not exceeding 45◦ . An increase in the applied voltage led to an increase of the coating roughness and porosity, thereby reducing the contact angles to 6◦ with water and to 17◦ with glycerol. The free surface energy of 75 ± 3 mJ/m2 for all the coatings were determined. Polar component was calculated as the main component of surface energy, caused by the presence of strong polar PO43− and OH− bonds. In vitro studies showed that low Cu and Zn amounts (~0.4 at.%) in the coatings promoted high motility of human adipose-derived multipotent mesenchymal stromal cells (hAMMSC) on the implant/cell interface and subsequent cell ability to differentiate into osteoblasts. In vivo study demonstrated 100% ectopic bone formation only on the surface of the CaP coating on Ti. The Zn-and Cu-containing CaP coatings on both substrates and the CaP coating on the Ti-40Nb alloy slightly decreased the incidence of ectopic osteogenesis down to 67%. The MAO coatings showed antibacterial efficacy against Staphylococcus aureus and can be arranged as follows: Zn-CaP/Ti > Cu-CaP/TiNb, Zn-CaP/TiNb > Cu-CaP/Ti.
AB - This work describes the wettability and biological performance of Zn-and Cu-containing CaP-based coatings prepared by micro-arc oxidation on pure titanium (Ti) and novel Ti-40Nb alloy. Good hydrophilic properties of all the coatings were demonstrated by the low contact angles with liquids, not exceeding 45◦ . An increase in the applied voltage led to an increase of the coating roughness and porosity, thereby reducing the contact angles to 6◦ with water and to 17◦ with glycerol. The free surface energy of 75 ± 3 mJ/m2 for all the coatings were determined. Polar component was calculated as the main component of surface energy, caused by the presence of strong polar PO43− and OH− bonds. In vitro studies showed that low Cu and Zn amounts (~0.4 at.%) in the coatings promoted high motility of human adipose-derived multipotent mesenchymal stromal cells (hAMMSC) on the implant/cell interface and subsequent cell ability to differentiate into osteoblasts. In vivo study demonstrated 100% ectopic bone formation only on the surface of the CaP coating on Ti. The Zn-and Cu-containing CaP coatings on both substrates and the CaP coating on the Ti-40Nb alloy slightly decreased the incidence of ectopic osteogenesis down to 67%. The MAO coatings showed antibacterial efficacy against Staphylococcus aureus and can be arranged as follows: Zn-CaP/Ti > Cu-CaP/TiNb, Zn-CaP/TiNb > Cu-CaP/Ti.
KW - Antibacterial efficacy
KW - Biocompatibility
KW - Bone lamellae
KW - Calcium phosphate coating
KW - Ectopic bone formation
KW - Micro-arc oxidation
KW - Ti-40wt.%Nb alloy
KW - Wettability
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U2 - 10.3390/ma13194366
DO - 10.3390/ma13194366
M3 - Article
AN - SCOPUS:85093926853
VL - 13
SP - 1
EP - 23
JO - Materials
JF - Materials
SN - 1996-1944
IS - 19
M1 - 4366
ER -