X-ray diffraction and biochemical studies of W34F mutant ribonuclease binase

K. M. Polyakov, D. A. Goncharuk, A. A. Trofimov, T. N. Safonova, V. A. Mitkevich, E. N. Tkach, A. A. Makarov, A. A. Shulga

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The structures of two crystal modifications of the W34F mutant ribonuclease from the bacterium Bacillus intermedius (binase) were solved and refined at 1.7 and 1.1 Å resolution. The kinetic parameters of the hydrolysis of substrates of different length (GpU, GpUp, and poly(I)) by binase and its W34F mutant were investigated and compared. The catalytic activity of the enzymes was shown to increase with increasing length of the substrate. The substitution of tryptophan for phenylalanine does not lead to a change in the activity of the enzyme but results in a decrease of the binding constants for substrates containing more than one phosphate groups. A comparison of the structure of the mutant enzyme with the previously established structures of binase and its complexes with sulfate ions and guanosine monophosphate showed that the difference in their kinetic parameters is related to the fact that the mutant ribonuclease cannot bind the second phosphate group. Both crystal modifications of the mutant binase contain dimers, like in the crystal structure of binase studied previously. In these dimers, only one enzyme molecule can bind the substrate molecule. Since the dimers were found in the crystals grown under four different conditions, it can be suggested that the enzyme can exist as dimers in solution as well. Mutants of binase, which could exclude the formation of dimers, are suggested.

Original languageEnglish
Pages (from-to)817-822
Number of pages6
JournalMolecular Biology
Volume44
Issue number5
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • binase
  • catalytic activity
  • ribonuclease
  • site-directed mutagenesis
  • three-dimensional structure

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology

Fingerprint Dive into the research topics of 'X-ray diffraction and biochemical studies of W34F mutant ribonuclease binase'. Together they form a unique fingerprint.

Cite this