Wharton's jelly Mesenchymal Stem Cell response on chitosan-graft-poly (ε-caprolactone) copolymer for myocardium tissue engineering

Maria Chatzinikolaidou, Maria Kaliva, Aristea Batsali, Charalampos Pontikoglou, Maria Vamvakaki

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Cell therapy and tissue engineering attract increasing attention as a potential approach for cardiac repair. Although a plethora of interesting concepts in the emerging field of cardiac stem cell-based tissue engineering are reported, there are still challenges that this field needs to overcome to achieve therapeutic translation into the clinical praxis. Engineering biomaterial scaffolds that facilitate stem cell engraftment, survival and homing are crucial for successful cellular cardiomyoplasty after myocardial infarction (MI). In this study we investigate for the first time the cellular response of Wharton's jelly (WJ) Mesenchymal Stem Cells (MSCs) on a copolymeric material comprising chitosan (CS) and poly(E-caprolactone) (PCL). First we synthesize a copolymer consisting of poly(£-caprolactone) grafted on a chemically modified chitosan-backbone (CS-g-PCL). Furthermore, we investigate the morphology, viability and proliferation of WJ-MSCs on material coatings and examine the cellular response from different donors. Our results show strong cell adhesion on the CS-g-PCL material surface from the first hours in culture, and a proliferation increase after 3 and 7 days. These findings support the potential use of our proposed cell-material combination in myocardium tissue engineering.

Original languageEnglish
Pages (from-to)2030-2039
Number of pages10
JournalCurrent Pharmaceutical Design
Volume20
Issue number12
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Cell adhesion
  • Chitosan grafted poly(e-caprolactone)
  • Myocardium regeneration
  • Proliferation
  • Viability
  • Wharton's jelly

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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