Water-soluble polysaccharides of herbs of suckling seeding medicago sativa (fabaceae) flora of the Krasnoyarsk territory

K. I. Rovkina, S. V. Krivoshchekov, A. M. Guryev, M. S. Yusubov, M. V. Belousov

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Alfalfa is a very high-quality perennial leguminous grass and has a long history of cultivation and application in traditional medicine in Russia. Alfalfa polysaccharides was extracted from alfalfa stems and leaves with distilled water in proportion to 1: 20 and was characterized with different methods. The uronic acid content of the polysaccharides was quantificated colorimetrically with modified carbazole – sulfuric method – 13,1 ± 0,9%. The protein content of the polysaccharides was quantificated with Lowry protein assay – 4,25 ± 0,28%. The nucleinic acids contents was quantificated with Spirin's method – 0,0056 ± 0,0003%. Ash-content (mineral impurities) was quantificated with gravimetrical method – 3,45 ± 0,18%. The description of IR spectrum was given. The monosaccharide composition of alfalfa polysaccharides was analysed by gas chromatography-mass spectrometry. The molecular weight was determined by high performance size-exclusion liquid chromatography. The crude alfalfa polysaccharides were fractionated by DEAE-cellulose chromatography. The sugar content of the fractions was assayed using the carbazole-sulphuric acid method. The alfalfa polysaccharides represents mix of seven polysaccharides.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalKhimiya Rastitel'nogo Syr'ya
Issue number2
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Alfalfa sowing
  • Medicago sativa
  • Polysaccharide complex

ASJC Scopus subject areas

  • Biomaterials
  • Plant Science
  • Organic Chemistry

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