Abstract
Alfalfa is a very high-quality perennial leguminous grass and has a long history of cultivation and application in traditional medicine in Russia. Alfalfa polysaccharides was extracted from alfalfa stems and leaves with distilled water in proportion to 1: 20 and was characterized with different methods. The uronic acid content of the polysaccharides was quantificated colorimetrically with modified carbazole – sulfuric method – 13,1 ± 0,9%. The protein content of the polysaccharides was quantificated with Lowry protein assay – 4,25 ± 0,28%. The nucleinic acids contents was quantificated with Spirin's method – 0,0056 ± 0,0003%. Ash-content (mineral impurities) was quantificated with gravimetrical method – 3,45 ± 0,18%. The description of IR spectrum was given. The monosaccharide composition of alfalfa polysaccharides was analysed by gas chromatography-mass spectrometry. The molecular weight was determined by high performance size-exclusion liquid chromatography. The crude alfalfa polysaccharides were fractionated by DEAE-cellulose chromatography. The sugar content of the fractions was assayed using the carbazole-sulphuric acid method. The alfalfa polysaccharides represents mix of seven polysaccharides.
| Original language | English |
|---|---|
| Pages (from-to) | 57-64 |
| Number of pages | 8 |
| Journal | Khimiya Rastitel'nogo Syr'ya |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Jan 2017 |
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Keywords
- Alfalfa sowing
- Medicago sativa
- Polysaccharide complex
ASJC Scopus subject areas
- Biomaterials
- Plant Science
- Organic Chemistry
Cite this
Water-soluble polysaccharides of herbs of suckling seeding medicago sativa (fabaceae) flora of the Krasnoyarsk territory. / Rovkina, K. I.; Krivoshchekov, S. V.; Guryev, A. M.; Yusubov, M. S.; Belousov, M. V.
In: Khimiya Rastitel'nogo Syr'ya, No. 2, 01.01.2017, p. 57-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Water-soluble polysaccharides of herbs of suckling seeding medicago sativa (fabaceae) flora of the Krasnoyarsk territory
AU - Rovkina, K. I.
AU - Krivoshchekov, S. V.
AU - Guryev, A. M.
AU - Yusubov, M. S.
AU - Belousov, M. V.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Alfalfa is a very high-quality perennial leguminous grass and has a long history of cultivation and application in traditional medicine in Russia. Alfalfa polysaccharides was extracted from alfalfa stems and leaves with distilled water in proportion to 1: 20 and was characterized with different methods. The uronic acid content of the polysaccharides was quantificated colorimetrically with modified carbazole – sulfuric method – 13,1 ± 0,9%. The protein content of the polysaccharides was quantificated with Lowry protein assay – 4,25 ± 0,28%. The nucleinic acids contents was quantificated with Spirin's method – 0,0056 ± 0,0003%. Ash-content (mineral impurities) was quantificated with gravimetrical method – 3,45 ± 0,18%. The description of IR spectrum was given. The monosaccharide composition of alfalfa polysaccharides was analysed by gas chromatography-mass spectrometry. The molecular weight was determined by high performance size-exclusion liquid chromatography. The crude alfalfa polysaccharides were fractionated by DEAE-cellulose chromatography. The sugar content of the fractions was assayed using the carbazole-sulphuric acid method. The alfalfa polysaccharides represents mix of seven polysaccharides.
AB - Alfalfa is a very high-quality perennial leguminous grass and has a long history of cultivation and application in traditional medicine in Russia. Alfalfa polysaccharides was extracted from alfalfa stems and leaves with distilled water in proportion to 1: 20 and was characterized with different methods. The uronic acid content of the polysaccharides was quantificated colorimetrically with modified carbazole – sulfuric method – 13,1 ± 0,9%. The protein content of the polysaccharides was quantificated with Lowry protein assay – 4,25 ± 0,28%. The nucleinic acids contents was quantificated with Spirin's method – 0,0056 ± 0,0003%. Ash-content (mineral impurities) was quantificated with gravimetrical method – 3,45 ± 0,18%. The description of IR spectrum was given. The monosaccharide composition of alfalfa polysaccharides was analysed by gas chromatography-mass spectrometry. The molecular weight was determined by high performance size-exclusion liquid chromatography. The crude alfalfa polysaccharides were fractionated by DEAE-cellulose chromatography. The sugar content of the fractions was assayed using the carbazole-sulphuric acid method. The alfalfa polysaccharides represents mix of seven polysaccharides.
KW - Alfalfa sowing
KW - Medicago sativa
KW - Polysaccharide complex
UR - http://www.scopus.com/inward/record.url?scp=85041585006&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85041585006&partnerID=8YFLogxK
U2 - 10.14258/jcprm.2017021231
DO - 10.14258/jcprm.2017021231
M3 - Article
SP - 57
EP - 64
JO - Khimiya Rastitel'nogo Syr'ya
JF - Khimiya Rastitel'nogo Syr'ya
SN - 1029-5151
IS - 2
ER -