Abstract
Alfalfa is a very high-quality perennial leguminous grass with a long history of cultivation and application in traditional medicine in Russia. In addition, alfalfa is a valuable forage representative of the legume family, its acreage in Russia reaches 2.3–2.5 million ha. The polysaccharide complex (PSC) was isolated from the aerial part of alfalfa by aqueous extraction (pH = 9) and characterized by physicochemical methods. The content of uronic acids was 13.1 ± 0.9%, the quantitative content of impurity substances such as protein was 4.25 ± 0.28%, nucleic acids 0.0056 ± 0.0003%, and mineral impurities (ash content) 3.45 ± 0.18%. The PSC was studied by IR spectroscopy. The monomeric composition of PSC was characterized by chromatography-mass spectrometry (major monosugars arabinose, glucose and galacturonic acid). The molecular weight distribution of PSC was studied by the high-pressure exclusion chromatography method. Data demonstrated that the polysaccharide complex of the aerial part of alfalfa consists of three components with molecular weights of 1100 ± 60, 16 ± 2 and 7 ± 1 kDa. The fact that PSC is a mixture of seven polysaccharides was demonstrated by the column ion-exchange chromatography method.
| Original language | English |
|---|---|
| Pages (from-to) | 854-859 |
| Number of pages | 6 |
| Journal | Russian Journal of Bioorganic Chemistry |
| Volume | 44 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Dec 2018 |
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Keywords
- alfalfa
- Medicago sativa
- polysaccharide complex
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry
Cite this
Water-Soluble Polysaccharides of Alfalfa (Medicago sativa (Fabaceae)) of Flora of Krasnoyarsk Krai. / Rovkina, K. I.; Krivoshchekov, S. V.; Guryev, A. M.; Yusubov, M. S.; Belousov, M. V.
In: Russian Journal of Bioorganic Chemistry, Vol. 44, No. 7, 01.12.2018, p. 854-859.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Water-Soluble Polysaccharides of Alfalfa (Medicago sativa (Fabaceae)) of Flora of Krasnoyarsk Krai
AU - Rovkina, K. I.
AU - Krivoshchekov, S. V.
AU - Guryev, A. M.
AU - Yusubov, M. S.
AU - Belousov, M. V.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Alfalfa is a very high-quality perennial leguminous grass with a long history of cultivation and application in traditional medicine in Russia. In addition, alfalfa is a valuable forage representative of the legume family, its acreage in Russia reaches 2.3–2.5 million ha. The polysaccharide complex (PSC) was isolated from the aerial part of alfalfa by aqueous extraction (pH = 9) and characterized by physicochemical methods. The content of uronic acids was 13.1 ± 0.9%, the quantitative content of impurity substances such as protein was 4.25 ± 0.28%, nucleic acids 0.0056 ± 0.0003%, and mineral impurities (ash content) 3.45 ± 0.18%. The PSC was studied by IR spectroscopy. The monomeric composition of PSC was characterized by chromatography-mass spectrometry (major monosugars arabinose, glucose and galacturonic acid). The molecular weight distribution of PSC was studied by the high-pressure exclusion chromatography method. Data demonstrated that the polysaccharide complex of the aerial part of alfalfa consists of three components with molecular weights of 1100 ± 60, 16 ± 2 and 7 ± 1 kDa. The fact that PSC is a mixture of seven polysaccharides was demonstrated by the column ion-exchange chromatography method.
AB - Alfalfa is a very high-quality perennial leguminous grass with a long history of cultivation and application in traditional medicine in Russia. In addition, alfalfa is a valuable forage representative of the legume family, its acreage in Russia reaches 2.3–2.5 million ha. The polysaccharide complex (PSC) was isolated from the aerial part of alfalfa by aqueous extraction (pH = 9) and characterized by physicochemical methods. The content of uronic acids was 13.1 ± 0.9%, the quantitative content of impurity substances such as protein was 4.25 ± 0.28%, nucleic acids 0.0056 ± 0.0003%, and mineral impurities (ash content) 3.45 ± 0.18%. The PSC was studied by IR spectroscopy. The monomeric composition of PSC was characterized by chromatography-mass spectrometry (major monosugars arabinose, glucose and galacturonic acid). The molecular weight distribution of PSC was studied by the high-pressure exclusion chromatography method. Data demonstrated that the polysaccharide complex of the aerial part of alfalfa consists of three components with molecular weights of 1100 ± 60, 16 ± 2 and 7 ± 1 kDa. The fact that PSC is a mixture of seven polysaccharides was demonstrated by the column ion-exchange chromatography method.
KW - alfalfa
KW - Medicago sativa
KW - polysaccharide complex
UR - http://www.scopus.com/inward/record.url?scp=85061772575&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061772575&partnerID=8YFLogxK
U2 - 10.1134/S1068162018070105
DO - 10.1134/S1068162018070105
M3 - Article
AN - SCOPUS:85061772575
VL - 44
SP - 854
EP - 859
JO - Russian Journal of Bioorganic Chemistry
JF - Russian Journal of Bioorganic Chemistry
SN - 1068-1620
IS - 7
ER -