Abstract
Present day biomedical applications, including magnetic biosensing, demand better understanding of the interactions between living systems and magnetic nanoparticles (MNPs). In this work spherical MNPs of maghemite were obtained by a highly productive laser target evaporation technique. XRD analysis confirmed the inverse spinel structure of the MNPs (space group Fd-3m). The ensemble obeyed a lognormal size distribution with the median value 26.8 nm and dispersion 0.362. Stabilized water-based suspensions were fabricated using electrostatic or steric stabilization by the natural polymer chitosan. The encapsulation of the MNPs by chitosan makes them resistant to the unfavorable factors for colloidal stability typically present in physiological conditions such as pH and high ionic force. Controlled amounts of suspensions were used for in vitro experiments with human blood mononuclear leukocytes (HBMLs) in order to study their morphofunctional response. For sake of comparison the results obtained in the present study were analyzed together with our previous results of the study of similar suspensions with human mesenchymal stem cells. Suspensions with and without chitosan enhanced the secretion of cytokines by a 24-h culture of HBMLs compared to a control without MNPs. At a dose of 2.3, the MTD of chitosan promotes the stimulating effect of MNPs on cells. In the dose range of MNPs 10-1000 MTD, chitosan “inhibits” cellular secretory activity compared to MNPs without chitosan. Both suspensions did not caused cell death by necrosis, hence, the secretion of cytokines is due to the enhancement of the functional activity of HBMLs. Increased accumulation of MNP with chitosan in the cell fraction at 100 MTD for 24 h exposure, may be due to fixation of chitosan on the outer membrane of HBMLs. The discussed results can be used for an addressed design of cell delivery/removal incorporating multiple activities because of cell capability to avoid phagocytosis by immune cells. They are also promising for the field of biosensor development for the detection of magnetic labels.
| Original language | English |
|---|---|
| Article number | 2605 |
| Journal | Sensors (Switzerland) |
| Volume | 17 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 13 Nov 2017 |
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Keywords
- Chitosan
- Ferrofluids
- Human blood mononuclear leukocytes
- Iron oxide magnetic nanoparticles
- Magnetic biosensors
- Morphofunctional response
ASJC Scopus subject areas
- Analytical Chemistry
- Atomic and Molecular Physics, and Optics
- Biochemistry
- Instrumentation
- Electrical and Electronic Engineering
Cite this
Water-Based suspensions of iron oxide nanoparticles with electrostatic or steric stabilization by chitosan : Fabrication, characterization and biocompatibility. / Kurlyandskaya, Galina V.; Litvinova, Larisa S.; Safronov, Alexander P.; Schupletsova, Valeria V.; Tyukova, Irina S.; Khaziakhmatova, Olga G.; Slepchenko, Galina B.; Yurova, Kristina A.; Cherempey, Elena G.; Kulesh, Nikita A.; Andrade, Ricardo; Beketov, Igor V.; Khlusov, Igor A.
In: Sensors (Switzerland), Vol. 17, No. 11, 2605, 13.11.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Water-Based suspensions of iron oxide nanoparticles with electrostatic or steric stabilization by chitosan
T2 - Fabrication, characterization and biocompatibility
AU - Kurlyandskaya, Galina V.
AU - Litvinova, Larisa S.
AU - Safronov, Alexander P.
AU - Schupletsova, Valeria V.
AU - Tyukova, Irina S.
AU - Khaziakhmatova, Olga G.
AU - Slepchenko, Galina B.
AU - Yurova, Kristina A.
AU - Cherempey, Elena G.
AU - Kulesh, Nikita A.
AU - Andrade, Ricardo
AU - Beketov, Igor V.
AU - Khlusov, Igor A.
PY - 2017/11/13
Y1 - 2017/11/13
N2 - Present day biomedical applications, including magnetic biosensing, demand better understanding of the interactions between living systems and magnetic nanoparticles (MNPs). In this work spherical MNPs of maghemite were obtained by a highly productive laser target evaporation technique. XRD analysis confirmed the inverse spinel structure of the MNPs (space group Fd-3m). The ensemble obeyed a lognormal size distribution with the median value 26.8 nm and dispersion 0.362. Stabilized water-based suspensions were fabricated using electrostatic or steric stabilization by the natural polymer chitosan. The encapsulation of the MNPs by chitosan makes them resistant to the unfavorable factors for colloidal stability typically present in physiological conditions such as pH and high ionic force. Controlled amounts of suspensions were used for in vitro experiments with human blood mononuclear leukocytes (HBMLs) in order to study their morphofunctional response. For sake of comparison the results obtained in the present study were analyzed together with our previous results of the study of similar suspensions with human mesenchymal stem cells. Suspensions with and without chitosan enhanced the secretion of cytokines by a 24-h culture of HBMLs compared to a control without MNPs. At a dose of 2.3, the MTD of chitosan promotes the stimulating effect of MNPs on cells. In the dose range of MNPs 10-1000 MTD, chitosan “inhibits” cellular secretory activity compared to MNPs without chitosan. Both suspensions did not caused cell death by necrosis, hence, the secretion of cytokines is due to the enhancement of the functional activity of HBMLs. Increased accumulation of MNP with chitosan in the cell fraction at 100 MTD for 24 h exposure, may be due to fixation of chitosan on the outer membrane of HBMLs. The discussed results can be used for an addressed design of cell delivery/removal incorporating multiple activities because of cell capability to avoid phagocytosis by immune cells. They are also promising for the field of biosensor development for the detection of magnetic labels.
AB - Present day biomedical applications, including magnetic biosensing, demand better understanding of the interactions between living systems and magnetic nanoparticles (MNPs). In this work spherical MNPs of maghemite were obtained by a highly productive laser target evaporation technique. XRD analysis confirmed the inverse spinel structure of the MNPs (space group Fd-3m). The ensemble obeyed a lognormal size distribution with the median value 26.8 nm and dispersion 0.362. Stabilized water-based suspensions were fabricated using electrostatic or steric stabilization by the natural polymer chitosan. The encapsulation of the MNPs by chitosan makes them resistant to the unfavorable factors for colloidal stability typically present in physiological conditions such as pH and high ionic force. Controlled amounts of suspensions were used for in vitro experiments with human blood mononuclear leukocytes (HBMLs) in order to study their morphofunctional response. For sake of comparison the results obtained in the present study were analyzed together with our previous results of the study of similar suspensions with human mesenchymal stem cells. Suspensions with and without chitosan enhanced the secretion of cytokines by a 24-h culture of HBMLs compared to a control without MNPs. At a dose of 2.3, the MTD of chitosan promotes the stimulating effect of MNPs on cells. In the dose range of MNPs 10-1000 MTD, chitosan “inhibits” cellular secretory activity compared to MNPs without chitosan. Both suspensions did not caused cell death by necrosis, hence, the secretion of cytokines is due to the enhancement of the functional activity of HBMLs. Increased accumulation of MNP with chitosan in the cell fraction at 100 MTD for 24 h exposure, may be due to fixation of chitosan on the outer membrane of HBMLs. The discussed results can be used for an addressed design of cell delivery/removal incorporating multiple activities because of cell capability to avoid phagocytosis by immune cells. They are also promising for the field of biosensor development for the detection of magnetic labels.
KW - Chitosan
KW - Ferrofluids
KW - Human blood mononuclear leukocytes
KW - Iron oxide magnetic nanoparticles
KW - Magnetic biosensors
KW - Morphofunctional response
UR - http://www.scopus.com/inward/record.url?scp=85034425587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034425587&partnerID=8YFLogxK
U2 - 10.3390/s17112605
DO - 10.3390/s17112605
M3 - Article
VL - 17
JO - Sensors
JF - Sensors
SN - 1424-3210
IS - 11
M1 - 2605
ER -