Abstract
Three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA)) have been determined by differential pulse voltammetry (DPV) at screen-printed carbon electrodes (SPCEs). As an optimum medium for all three analytes, Britton-Robinson buffer (0.04 mol ⋅ L−1, pH 3.0) was used. Dependencies of the peak current on the concentration of a biomarker were linear in the concentration range from 0.2 to 100 μmol ⋅ L−1 for all the tested compounds, with the limits of quantification 0.8 μmol ⋅ L−1 for homovanillic acid, 0.2 μmol ⋅ L−1 for vanillylmandelic acid, and 0.4 μmol ⋅ L−1 for 5-hydroxyindole-3-acetic acid. Moreover, it was verified that the newly developed methods can be used for simultaneous determination of HVA and VMA in mixture.
| Original language | English |
|---|---|
| Pages (from-to) | 146-153 |
| Number of pages | 8 |
| Journal | Electroanalysis |
| Volume | 29 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2017 |
| Externally published | Yes |
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Keywords
- 5-Hydroxyindole-3-acetic acid
- Differential pulse voltammetry
- Homovanillic acid
- Screen-printed carbon electrodes
- Vanillylmandelic acid
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Voltammetric Determination of Tumor Biomarkers for Neuroblastoma (Homovanillic Acid, Vanillylmandelic Acid, and 5-Hydroxyindole-3-acetic Acid) at Screen-printed Carbon Electrodes. / Makrlíková, Anna; Ktena, Evangelia; Economou, Anastasios; Fischer, Jan; Navrátil, Tomáš; Barek, Jiří; Vyskočil, Vlastimil.
In: Electroanalysis, Vol. 29, No. 1, 01.01.2017, p. 146-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Voltammetric Determination of Tumor Biomarkers for Neuroblastoma (Homovanillic Acid, Vanillylmandelic Acid, and 5-Hydroxyindole-3-acetic Acid) at Screen-printed Carbon Electrodes
AU - Makrlíková, Anna
AU - Ktena, Evangelia
AU - Economou, Anastasios
AU - Fischer, Jan
AU - Navrátil, Tomáš
AU - Barek, Jiří
AU - Vyskočil, Vlastimil
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA)) have been determined by differential pulse voltammetry (DPV) at screen-printed carbon electrodes (SPCEs). As an optimum medium for all three analytes, Britton-Robinson buffer (0.04 mol ⋅ L−1, pH 3.0) was used. Dependencies of the peak current on the concentration of a biomarker were linear in the concentration range from 0.2 to 100 μmol ⋅ L−1 for all the tested compounds, with the limits of quantification 0.8 μmol ⋅ L−1 for homovanillic acid, 0.2 μmol ⋅ L−1 for vanillylmandelic acid, and 0.4 μmol ⋅ L−1 for 5-hydroxyindole-3-acetic acid. Moreover, it was verified that the newly developed methods can be used for simultaneous determination of HVA and VMA in mixture.
AB - Three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA)) have been determined by differential pulse voltammetry (DPV) at screen-printed carbon electrodes (SPCEs). As an optimum medium for all three analytes, Britton-Robinson buffer (0.04 mol ⋅ L−1, pH 3.0) was used. Dependencies of the peak current on the concentration of a biomarker were linear in the concentration range from 0.2 to 100 μmol ⋅ L−1 for all the tested compounds, with the limits of quantification 0.8 μmol ⋅ L−1 for homovanillic acid, 0.2 μmol ⋅ L−1 for vanillylmandelic acid, and 0.4 μmol ⋅ L−1 for 5-hydroxyindole-3-acetic acid. Moreover, it was verified that the newly developed methods can be used for simultaneous determination of HVA and VMA in mixture.
KW - 5-Hydroxyindole-3-acetic acid
KW - Differential pulse voltammetry
KW - Homovanillic acid
KW - Screen-printed carbon electrodes
KW - Vanillylmandelic acid
UR - http://www.scopus.com/inward/record.url?scp=85009289946&partnerID=8YFLogxK
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U2 - 10.1002/elan.201600534
DO - 10.1002/elan.201600534
M3 - Article
VL - 29
SP - 146
EP - 153
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1040-0397
IS - 1
ER -