TY - JOUR
T1 - Voltammetric Determination of Tumor Biomarkers for Neuroblastoma (Homovanillic Acid, Vanillylmandelic Acid, and 5-Hydroxyindole-3-acetic Acid) at Screen-printed Carbon Electrodes
AU - Makrlíková, Anna
AU - Ktena, Evangelia
AU - Economou, Anastasios
AU - Fischer, Jan
AU - Navrátil, Tomáš
AU - Barek, Jiří
AU - Vyskočil, Vlastimil
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA)) have been determined by differential pulse voltammetry (DPV) at screen-printed carbon electrodes (SPCEs). As an optimum medium for all three analytes, Britton-Robinson buffer (0.04 mol ⋅ L−1, pH 3.0) was used. Dependencies of the peak current on the concentration of a biomarker were linear in the concentration range from 0.2 to 100 μmol ⋅ L−1 for all the tested compounds, with the limits of quantification 0.8 μmol ⋅ L−1 for homovanillic acid, 0.2 μmol ⋅ L−1 for vanillylmandelic acid, and 0.4 μmol ⋅ L−1 for 5-hydroxyindole-3-acetic acid. Moreover, it was verified that the newly developed methods can be used for simultaneous determination of HVA and VMA in mixture.
AB - Three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA)) have been determined by differential pulse voltammetry (DPV) at screen-printed carbon electrodes (SPCEs). As an optimum medium for all three analytes, Britton-Robinson buffer (0.04 mol ⋅ L−1, pH 3.0) was used. Dependencies of the peak current on the concentration of a biomarker were linear in the concentration range from 0.2 to 100 μmol ⋅ L−1 for all the tested compounds, with the limits of quantification 0.8 μmol ⋅ L−1 for homovanillic acid, 0.2 μmol ⋅ L−1 for vanillylmandelic acid, and 0.4 μmol ⋅ L−1 for 5-hydroxyindole-3-acetic acid. Moreover, it was verified that the newly developed methods can be used for simultaneous determination of HVA and VMA in mixture.
KW - 5-Hydroxyindole-3-acetic acid
KW - Differential pulse voltammetry
KW - Homovanillic acid
KW - Screen-printed carbon electrodes
KW - Vanillylmandelic acid
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U2 - 10.1002/elan.201600534
DO - 10.1002/elan.201600534
M3 - Article
AN - SCOPUS:85009289946
VL - 29
SP - 146
EP - 153
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1040-0397
IS - 1
ER -