Voltametrické stanovení stopových množství 2-aminofluoren-9-onu s předřazenou prekoncentrací analytu pomocí extrakce na tuhou fázi

Translated title of the contribution: Voltammetric determination of trace amounts of 2-aminofluoren- 9-one with preconcentration of the analyte by solid-phase extraction

Andrea Hájková, Vlastimil Vyskočil, Jiří Barek

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Optimum conditions were found for the determination of nanomolar and subnanomolar concentrations of genotoxic 2-aminofluoren-9-one (2-AFN) by adsorptive stripping differential pulse voltammetry (AdSDPV) at a hanging mercury drop minielectrode (HMDmE) after preliminary separation and preconcentration by solidphase extraction (SPE) in LiChrolut RP-18 E (500 mg) columns. The adsorbed analyte was eluted with acetone, the solution then evaporated to dryness, and the residue dissolved in acetate buffer (pH 4.0) (AcB). A sample was then measured by AdSDPV at the HMDmE, with the accumulation potential 100 mV (vs. Ag{pipe}AgCl in 1 mol l-1 KCl) and the accumulation time 120 s. Linear calibration curves were obtained for 2·10-11 - 1·10-9 mol l-1 2-AFN, with the limit of quantification (LQ) ≈ 4·10-11 mol l-1. The method was verified by the determination of 2-AFN in model samples of drinking and river water at concentrations 4·10-11 - 1·10-9 and 2·10-10 - 1·10-9 mol l-1 (LQ ≈ 6·10-11 and 4·10-10 mol l-1, respectively).

Original languageCzech
Pages (from-to)234-240
Number of pages7
JournalChemicke Listy
Volume107
Issue number3
Publication statusPublished - 1 Mar 2013
Externally publishedYes

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Voltammetry
Mercury
Acetone
Buffers
Acetates
Rivers
Pipe
Calibration
Water

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Voltametrické stanovení stopových množství 2-aminofluoren-9-onu s předřazenou prekoncentrací analytu pomocí extrakce na tuhou fázi. / Hájková, Andrea; Vyskočil, Vlastimil; Barek, Jiří.

In: Chemicke Listy, Vol. 107, No. 3, 01.03.2013, p. 234-240.

Research output: Contribution to journalArticle

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AB - Optimum conditions were found for the determination of nanomolar and subnanomolar concentrations of genotoxic 2-aminofluoren-9-one (2-AFN) by adsorptive stripping differential pulse voltammetry (AdSDPV) at a hanging mercury drop minielectrode (HMDmE) after preliminary separation and preconcentration by solidphase extraction (SPE) in LiChrolut RP-18 E (500 mg) columns. The adsorbed analyte was eluted with acetone, the solution then evaporated to dryness, and the residue dissolved in acetate buffer (pH 4.0) (AcB). A sample was then measured by AdSDPV at the HMDmE, with the accumulation potential 100 mV (vs. Ag{pipe}AgCl in 1 mol l-1 KCl) and the accumulation time 120 s. Linear calibration curves were obtained for 2·10-11 - 1·10-9 mol l-1 2-AFN, with the limit of quantification (LQ) ≈ 4·10-11 mol l-1. The method was verified by the determination of 2-AFN in model samples of drinking and river water at concentrations 4·10-11 - 1·10-9 and 2·10-10 - 1·10-9 mol l-1 (LQ ≈ 6·10-11 and 4·10-10 mol l-1, respectively).

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