Abstract
Optimum conditions were found for the determination of nanomolar and subnanomolar concentrations of genotoxic 2-aminofluoren-9-one (2-AFN) by adsorptive stripping differential pulse voltammetry (AdSDPV) at a hanging mercury drop minielectrode (HMDmE) after preliminary separation and preconcentration by solidphase extraction (SPE) in LiChrolut RP-18 E (500 mg) columns. The adsorbed analyte was eluted with acetone, the solution then evaporated to dryness, and the residue dissolved in acetate buffer (pH 4.0) (AcB). A sample was then measured by AdSDPV at the HMDmE, with the accumulation potential 100 mV (vs. Ag{pipe}AgCl in 1 mol l-1 KCl) and the accumulation time 120 s. Linear calibration curves were obtained for 2·10-11 - 1·10-9 mol l-1 2-AFN, with the limit of quantification (LQ) ≈ 4·10-11 mol l-1. The method was verified by the determination of 2-AFN in model samples of drinking and river water at concentrations 4·10-11 - 1·10-9 and 2·10-10 - 1·10-9 mol l-1 (LQ ≈ 6·10-11 and 4·10-10 mol l-1, respectively).
| Original language | Czech |
|---|---|
| Pages (from-to) | 234-240 |
| Number of pages | 7 |
| Journal | Chemicke Listy |
| Volume | 107 |
| Issue number | 3 |
| Publication status | Published - 1 Mar 2013 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Chemistry(all)
Cite this
Voltametrické stanovení stopových množství 2-aminofluoren-9-onu s předřazenou prekoncentrací analytu pomocí extrakce na tuhou fázi. / Hájková, Andrea; Vyskočil, Vlastimil; Barek, Jiří.
In: Chemicke Listy, Vol. 107, No. 3, 01.03.2013, p. 234-240.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Voltametrické stanovení stopových množství 2-aminofluoren-9-onu s předřazenou prekoncentrací analytu pomocí extrakce na tuhou fázi
AU - Hájková, Andrea
AU - Vyskočil, Vlastimil
AU - Barek, Jiří
PY - 2013/3/1
Y1 - 2013/3/1
N2 - Optimum conditions were found for the determination of nanomolar and subnanomolar concentrations of genotoxic 2-aminofluoren-9-one (2-AFN) by adsorptive stripping differential pulse voltammetry (AdSDPV) at a hanging mercury drop minielectrode (HMDmE) after preliminary separation and preconcentration by solidphase extraction (SPE) in LiChrolut RP-18 E (500 mg) columns. The adsorbed analyte was eluted with acetone, the solution then evaporated to dryness, and the residue dissolved in acetate buffer (pH 4.0) (AcB). A sample was then measured by AdSDPV at the HMDmE, with the accumulation potential 100 mV (vs. Ag{pipe}AgCl in 1 mol l-1 KCl) and the accumulation time 120 s. Linear calibration curves were obtained for 2·10-11 - 1·10-9 mol l-1 2-AFN, with the limit of quantification (LQ) ≈ 4·10-11 mol l-1. The method was verified by the determination of 2-AFN in model samples of drinking and river water at concentrations 4·10-11 - 1·10-9 and 2·10-10 - 1·10-9 mol l-1 (LQ ≈ 6·10-11 and 4·10-10 mol l-1, respectively).
AB - Optimum conditions were found for the determination of nanomolar and subnanomolar concentrations of genotoxic 2-aminofluoren-9-one (2-AFN) by adsorptive stripping differential pulse voltammetry (AdSDPV) at a hanging mercury drop minielectrode (HMDmE) after preliminary separation and preconcentration by solidphase extraction (SPE) in LiChrolut RP-18 E (500 mg) columns. The adsorbed analyte was eluted with acetone, the solution then evaporated to dryness, and the residue dissolved in acetate buffer (pH 4.0) (AcB). A sample was then measured by AdSDPV at the HMDmE, with the accumulation potential 100 mV (vs. Ag{pipe}AgCl in 1 mol l-1 KCl) and the accumulation time 120 s. Linear calibration curves were obtained for 2·10-11 - 1·10-9 mol l-1 2-AFN, with the limit of quantification (LQ) ≈ 4·10-11 mol l-1. The method was verified by the determination of 2-AFN in model samples of drinking and river water at concentrations 4·10-11 - 1·10-9 and 2·10-10 - 1·10-9 mol l-1 (LQ ≈ 6·10-11 and 4·10-10 mol l-1, respectively).
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M3 - Статья
VL - 107
SP - 234
EP - 240
JO - Chemicke Listy
JF - Chemicke Listy
SN - 0009-2770
IS - 3
ER -