Voltammetric detection of damage to DNA caused by nitro derivatives of fluorene using an electrochemical DNA biosensor

Vlastimil Vyskočil, Ján Labuda, Jiří Barek

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

An electrochemical DNA biosensor based on the screen printed carbon paste electrode (SPCPE) with an immobilized layer of calf thymus double-stranded DNA has been used for in vitro investigation of the interaction between genotoxic nitro derivatives of fluorene (namely 2-nitrofluorene and 2,7-dinitrofluorene) and DNA. Two types of DNA damage have been detected at the DNA/SPCPE biosensor: first, that caused by direct association of the nitrofluorenes, for which an intercalation association has been found using the known DNA intercalators [Cu(phen)2]2+ and [Co(phen)3]3+ as competing agents, and, second, that caused by short-lived radicals generated by electrochemical reduction of the nitro group (observable under specific conditions only).

Original languageEnglish
Pages (from-to)233-241
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume397
Issue number1
DOIs
Publication statusPublished - 1 May 2010
Externally publishedYes

Keywords

  • 2,7-Dinitrofluorene
  • 2-Nitrofluorene
  • DNA biosensor
  • DNA damage
  • Electrochemical detection
  • Screen printed carbon paste electrode

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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