Voltammetric Detection of Catecholamine Metabolites Using Tröger's Base Modified Electrode

Tatiana V. Shishkanova, Gabriela Broncová, Přemysl Fitl, Vladimír Král, Jiří Barek

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The advantage of surface modification of voltammetric sensor for determination of catecholamine metabolites such as vanillyl mandelic acid (VMA) and homovanillic acid (HVA) is discussed. The amino-substituted coumarin derivative of Tröger's base was deposi-ted on a graphite screen-printed electrode (poly(CTB)/G/SPE) cycling the electrode potential between 0 and 1.2 V in 5 mL CH2Cl2 containing 1×10−3 mol L−1 tetrabutylammonium tetrafluoroborate and 20 μL 0.1 mol L−1 HCl. The poly(CTB) functions as both a selective recognition and pre-concentrator agent for catecholamine metabolites. The high sensitivity of the voltammetric sensor coated by a poly(CTB) film toward VMA was achieved despite the high structural similarity between the two tested metabolites. We presume the voltammetric detection of these species is based on selective binding by a poly(CTB) film supported by its good adherence to the electrode substrate. The voltammetric signals of VMA were observable in the concentration range 10–100 μmol L−1 (LOD=0.2 μmol L−1) and 20–60 μmol L−1 (LOD=10 μmol L−1) in the model and synthethic urine samples, respectively.

Original languageEnglish
Pages (from-to)734-739
Number of pages6
JournalElectroanalysis
Volume30
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018
Externally publishedYes

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Keywords

  • homovanillic acid
  • polymeric Tröger's base
  • vanillyl mandelic acid
  • voltammetric sensor

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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