Voltammetric and amperometric determination of n-nitroso antineoplastic drugs at mercury and amalgam electrodes

Karolina Pecková, Lucie Vrzalová, Vladimír Bencko, Jiří Barek

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

A hanging mercury drop electrode and a mercury meniscus modified silver solid amalgam electrode were used as electroanalytical sensors for voltammetric determination of antineoplastic drugs carmustine, lomustine and streptozotocin containing reducible N-nitroso groups. On the example of carmustine it was shown that its one-step reduction proceeds at substantially more negative potentials at amalgam electrode as compared with mercury electrode. Both electrodes offer satisfactory repeatability of current response (relative standard deviations < 5%) using DC voltammetry and differential pulse voltammetry. The achieved limits of determination lie mostly in the 10-7 mol l-1 concentration range. The mentioned voltammetric methods were applied to determination of carmustine and lomustine in pharmaceutical formulations. Further, the mercury meniscus modified silver solid amalgam electrode was employed in a "wall-jet" amperometric detection cell in the determination of carmustine by flow injection analysis. Under optimized conditions (run electrolyte Britton-Robinson buffer of pH 7.0; flow rate 5.5 ml min-1; detection potential-1.5 V; injection volume 0.02 ml) the limit of quantitation 7.1 × 10-6 mol l-1 was achieved.

Original languageEnglish
Pages (from-to)1697-1713
Number of pages17
JournalCollection of Czechoslovak Chemical Communications
Volume74
Issue number11-12
DOIs
Publication statusPublished - 1 Nov 2009
Externally publishedYes

Keywords

  • Amperometry
  • Carmustine
  • Electrochemistry
  • Hanging mercury drop electrode
  • Lomustine
  • Nitroso compounds
  • Silver solid amalgam electrode
  • Streptozotocin
  • Voltammetry

ASJC Scopus subject areas

  • Chemistry(all)

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