Methods for determination of 2-amino-4-nitrophenol and 4-amino-2- nitrophenol, metabolites of 2,4-dinitrophenol, were developed using differential pulse (DP) voltammetry and HPLC with amperometric and spectrophotometric detection. The applicability of these methods was tested by the determination of the analytes in model samples of urine after preliminary separation by solid-phase extraction. Voltammetry enabled parallel determination of both analytes, but its application in real matrix was severely limited due to the interference of other compounds present in urine. HPLC allowed the determination in real urine matrix down to micromolar concentrations; amperometric detection proved to be more sensitive and selective than the spectrophotometric one.
- 2,4-Dinitrophenol metabolites
- Carbon paste electrode
- Differential pulse voltammetry
- HPLC with amperometric detection
ASJC Scopus subject areas
- Analytical Chemistry