UV excimer lamp irradiation of fibroblasts

The influence on antioxidant homeostasis

Michael V. Erofeev, Ingrid E. Kieft, Edward A. Sosnin, Eva Stoffels

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

UV excimer lamps are efficient and economical narrowband sources of UVB and UVC radiation. Potentially, these lamps may be used in bacterial disinfection or in minor cosmetic procedures, but safety tests are necessary. For this purpose, cellular damage induced by the UV radiation is assessed. 3T3 mouse fibroblasts are used as a model system. It is found that lethal damage occurs above a certain wavelength-dependent threshold dose. Furthermore, the antioxidant balance of the cells is influenced; glutathione levels are usually depleted, both under and above the lethal threshold. A striking increase of glutathione level is observed after UVC irradiation. This is attributed to an enhanced repair activity as a response to acute oxidative stress induced by UVC. In contrast to UVC, low-power irradiation with UVB seems to induce very little cellular damage.

Original languageEnglish
Pages (from-to)1359-1364
Number of pages6
JournalIEEE Transactions on Plasma Science
Volume34
Issue number4 II
DOIs
Publication statusPublished - 1 Aug 2006

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homeostasis
fibroblasts
antioxidants
excimers
luminaires
glutathione
damage
irradiation
thresholds
radiation
mice
narrowband
safety
dosage
cells
wavelengths

Keywords

  • Damage
  • Excilamp
  • Fibroblast
  • Oxidative stress
  • UV exciplex lamp

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Condensed Matter Physics

Cite this

UV excimer lamp irradiation of fibroblasts : The influence on antioxidant homeostasis. / Erofeev, Michael V.; Kieft, Ingrid E.; Sosnin, Edward A.; Stoffels, Eva.

In: IEEE Transactions on Plasma Science, Vol. 34, No. 4 II, 01.08.2006, p. 1359-1364.

Research output: Contribution to journalArticle

Erofeev, Michael V. ; Kieft, Ingrid E. ; Sosnin, Edward A. ; Stoffels, Eva. / UV excimer lamp irradiation of fibroblasts : The influence on antioxidant homeostasis. In: IEEE Transactions on Plasma Science. 2006 ; Vol. 34, No. 4 II. pp. 1359-1364.
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