Abstract
Surface-enhanced Raman scattering (SERS) has become an attractive analytical tool for recognition of various biomolecules and bio-objects. In this work, we created a universal platform, based on surface modified gold multibranched nanoparticles (AuMs), plasmon coupling between surface plasmon polariton waves (SPP) and localized surface plasmon (LSP), and utilization of advanced statistical methods for analysis and estimation of SERS spectra, measured on the "tumor" cells cultivation media. At the first stage, we used the AuMs which have an extremely high number of plasmonic hot spots per single nanoparticle and provide excellent SERS signal intensification. The surface of AuMs was decorated with charged organic functional moieties to entrap various (bio) molecules from targeted solution. Chemical and morphological structures of the prepared nanoparticles were examined with different techniques such as SEM-EDX and Raman spectroscopy. The decorated AuMs were placed in the cultivation media of melanoma and fibroblast lines in vitro. After interaction with cultivation media the AuMs were drop-deposited on the periodical gold grating, to achieve SPP-LSP coupling and even more enhance the SERS signal from entrapped (bio)molecules. Raman spectra were collected from several kinds of melanoma cells lines and evaluated using the developed mathematical route.
| Original language | English |
|---|---|
| Title of host publication | NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application |
| Publisher | TANGER Ltd. |
| Pages | 354-359 |
| Number of pages | 6 |
| ISBN (Electronic) | 9788087294895 |
| Publication status | Published - 1 Jan 2019 |
| Event | 10th Anniversary International Conference on Nanomaterials - Research and Application, NANOCON 2018 - Brno, Czech Republic Duration: 17 Oct 2018 → 19 Oct 2018 |
Publication series
| Name | NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application |
|---|
Conference
| Conference | 10th Anniversary International Conference on Nanomaterials - Research and Application, NANOCON 2018 |
|---|---|
| Country | Czech Republic |
| City | Brno |
| Period | 17.10.18 → 19.10.18 |
Fingerprint
Keywords
- Arenediazonium tosylates
- Cultivation media
- Gold nanoparticles
- SERS detection
ASJC Scopus subject areas
- Biotechnology
- Electrical and Electronic Engineering
- Ceramics and Composites
- Electronic, Optical and Magnetic Materials
Cite this
Universal platform for sers detection of "normal" and "tumor" cells cultivation media. / Erzina, Mariia; Trelin, Andrii; Guselnikova, Olga; Kalachyova, Yevgeniya; Perminova, Anastasia; Postnikov, Pavel; Vorcik, Vaclac; Lyutakov, Oleksiy.
NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application. TANGER Ltd., 2019. p. 354-359 (NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Universal platform for sers detection of "normal" and "tumor" cells cultivation media
AU - Erzina, Mariia
AU - Trelin, Andrii
AU - Guselnikova, Olga
AU - Kalachyova, Yevgeniya
AU - Perminova, Anastasia
AU - Postnikov, Pavel
AU - Vorcik, Vaclac
AU - Lyutakov, Oleksiy
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Surface-enhanced Raman scattering (SERS) has become an attractive analytical tool for recognition of various biomolecules and bio-objects. In this work, we created a universal platform, based on surface modified gold multibranched nanoparticles (AuMs), plasmon coupling between surface plasmon polariton waves (SPP) and localized surface plasmon (LSP), and utilization of advanced statistical methods for analysis and estimation of SERS spectra, measured on the "tumor" cells cultivation media. At the first stage, we used the AuMs which have an extremely high number of plasmonic hot spots per single nanoparticle and provide excellent SERS signal intensification. The surface of AuMs was decorated with charged organic functional moieties to entrap various (bio) molecules from targeted solution. Chemical and morphological structures of the prepared nanoparticles were examined with different techniques such as SEM-EDX and Raman spectroscopy. The decorated AuMs were placed in the cultivation media of melanoma and fibroblast lines in vitro. After interaction with cultivation media the AuMs were drop-deposited on the periodical gold grating, to achieve SPP-LSP coupling and even more enhance the SERS signal from entrapped (bio)molecules. Raman spectra were collected from several kinds of melanoma cells lines and evaluated using the developed mathematical route.
AB - Surface-enhanced Raman scattering (SERS) has become an attractive analytical tool for recognition of various biomolecules and bio-objects. In this work, we created a universal platform, based on surface modified gold multibranched nanoparticles (AuMs), plasmon coupling between surface plasmon polariton waves (SPP) and localized surface plasmon (LSP), and utilization of advanced statistical methods for analysis and estimation of SERS spectra, measured on the "tumor" cells cultivation media. At the first stage, we used the AuMs which have an extremely high number of plasmonic hot spots per single nanoparticle and provide excellent SERS signal intensification. The surface of AuMs was decorated with charged organic functional moieties to entrap various (bio) molecules from targeted solution. Chemical and morphological structures of the prepared nanoparticles were examined with different techniques such as SEM-EDX and Raman spectroscopy. The decorated AuMs were placed in the cultivation media of melanoma and fibroblast lines in vitro. After interaction with cultivation media the AuMs were drop-deposited on the periodical gold grating, to achieve SPP-LSP coupling and even more enhance the SERS signal from entrapped (bio)molecules. Raman spectra were collected from several kinds of melanoma cells lines and evaluated using the developed mathematical route.
KW - Arenediazonium tosylates
KW - Cultivation media
KW - Gold nanoparticles
KW - SERS detection
UR - http://www.scopus.com/inward/record.url?scp=85062971722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062971722&partnerID=8YFLogxK
M3 - Conference contribution
T3 - NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application
SP - 354
EP - 359
BT - NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application
PB - TANGER Ltd.
ER -