Universal platform for sers detection of "normal" and "tumor" cells cultivation media

Mariia Erzina, Andrii Trelin, Olga Guselnikova, Yevgeniya Kalachyova, Anastasia Perminova, Pavel Postnikov, Vaclac Vorcik, Oleksiy Lyutakov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Surface-enhanced Raman scattering (SERS) has become an attractive analytical tool for recognition of various biomolecules and bio-objects. In this work, we created a universal platform, based on surface modified gold multibranched nanoparticles (AuMs), plasmon coupling between surface plasmon polariton waves (SPP) and localized surface plasmon (LSP), and utilization of advanced statistical methods for analysis and estimation of SERS spectra, measured on the "tumor" cells cultivation media. At the first stage, we used the AuMs which have an extremely high number of plasmonic hot spots per single nanoparticle and provide excellent SERS signal intensification. The surface of AuMs was decorated with charged organic functional moieties to entrap various (bio) molecules from targeted solution. Chemical and morphological structures of the prepared nanoparticles were examined with different techniques such as SEM-EDX and Raman spectroscopy. The decorated AuMs were placed in the cultivation media of melanoma and fibroblast lines in vitro. After interaction with cultivation media the AuMs were drop-deposited on the periodical gold grating, to achieve SPP-LSP coupling and even more enhance the SERS signal from entrapped (bio)molecules. Raman spectra were collected from several kinds of melanoma cells lines and evaluated using the developed mathematical route.

Original languageEnglish
Title of host publicationNANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application
PublisherTANGER Ltd.
Pages354-359
Number of pages6
ISBN (Electronic)9788087294895
Publication statusPublished - 1 Jan 2019
Event10th Anniversary International Conference on Nanomaterials - Research and Application, NANOCON 2018 - Brno, Czech Republic
Duration: 17 Oct 201819 Oct 2018

Publication series

NameNANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application

Conference

Conference10th Anniversary International Conference on Nanomaterials - Research and Application, NANOCON 2018
CountryCzech Republic
CityBrno
Period17.10.1819.10.18

Keywords

  • Arenediazonium tosylates
  • Cultivation media
  • Gold nanoparticles
  • SERS detection

ASJC Scopus subject areas

  • Biotechnology
  • Electrical and Electronic Engineering
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials

Fingerprint Dive into the research topics of 'Universal platform for sers detection of "normal" and "tumor" cells cultivation media'. Together they form a unique fingerprint.

  • Cite this

    Erzina, M., Trelin, A., Guselnikova, O., Kalachyova, Y., Perminova, A., Postnikov, P., Vorcik, V., & Lyutakov, O. (2019). Universal platform for sers detection of "normal" and "tumor" cells cultivation media. In NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application (pp. 354-359). (NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application). TANGER Ltd..