Ubiquitous and cell type-specific transcriptomic changes triggered by dissipation of monovalent cation gradients in rodent cells: Physiological and pathophysiological implications

Elizaveta A. Klimanova, Svetlana V. Sidorenko, Larisa V. Smolyaninova, Leonid V. Kapilevich, Svetlana V. Gusakova, Olga D. Lopina, Sergei N. Orlov

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

Elevation of [Na+]i/[K+]i-ratio is considered as one of the major signals triggering transcriptomic changes in various cells types. In this study, we identified ubiquitous and cell type-specific Na+ i,K+ i-sensitive genes by comparative analysis of transcriptomic changes in ouabain-treated rat aorta smooth muscle cells and rat aorta endothelial cells (RASMC and RAEC, respectively), rat cerebellar granule cells (RCGC), and mouse C2C12 myoblasts. Exposure of the cells to ouabain increased intracellular Na+ content by ~ 14, 8, 7, and 6-fold and resulted in appearance of 7577, 2698, 2120, and 1146 differentially expressed transcripts in RAEC, RASMC, C2C12, and RCGC, respectively. Eighty-three genes were found as the intersection of the four sets of identified transcripts corresponding to each cell type and are classified as ubiquitous. Among the 10 top upregulated ubiquitous transcripts are the following: Dusp6, Plk3, Trib1, Ccl7, Mafk, Atf3, Ptgs2, Cxcl1, Spry4, and Coq10b. Unique transcripts whose expression is cell-specific include 4897, 1523, 789, and 494 transcripts for RAEC, RASMC, C2C12, and RCGC, respectively. The role of gene expression and signal pathways induced by dissipation of transmembrane gradient of monovalent cations in the development of various diseases is discussed with special attention to cardiovascular and pulmonary illnesses.

Original languageEnglish
Title of host publicationCurrent Topics in Membranes
EditorsSergei N. Orlov
PublisherAcademic Press Inc.
Pages107-149
Number of pages43
ISBN (Print)9780128177648
DOIs
Publication statusPublished - 2019

Publication series

NameCurrent Topics in Membranes
Volume83
ISSN (Print)1063-5823

Keywords

  • Excitation-transcription coupling
  • Intracellular potassium
  • Intracellular sodium
  • Microarray analysis
  • Na,K-ATPase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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