Two-stage implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts induced into osteoblast differentiation for direct skeletal attachment of limb prostheses

Maxim A. Shevtsov, Oleg V. Galibin, Nataliya M. Yudintceva, Miralda I. Blinova, George P. Pinaev, Anna A. Ivanova, Olga N. Savchenko, Dmitriy N. Suslov, Igor L. Potokin, Emil Pitkin, Grigory Raykhtsaum, Mark R. Pitkin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Angio- and osteogenesis following the two-stage (TS) implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts was evaluated. Two consecutive animal substudies were undertaken: intramedullary subcutaneous implantation (15 rabbits) and a TS transcutaneous implantation (12 rabbits). We observed enhanced osseointegrative properties of the intramedullary porous component seeded with fibroblasts induced into osteoblast differentiation, as compared to the untreated porous titanium pylon. The three-phase scintigraphy and subsequent histological analysis showed that the level of osteogenesis was 1.5-fold higher than in the control group, and significantly so (p < 0.05). The biocompatibility was further proved by the absence of inflammatory response or encapsulation and sequestration on the histology assay. Treatment of the transcutaneous component with autologous fibroblasts was associated with nearly a 2-fold decrease in the period required for the ingrowth of dermal and subdermal soft tissues into the implant surface, as compared to the untreated porous titanium component. Direct dermal attachment to the transcutaneous implant prevented superficial and deep periprosthetic infections in rabbits in vivo.

Original languageEnglish
Pages (from-to)3033-3048
Number of pages16
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number9
DOIs
Publication statusPublished - 1 Jan 2014

Fingerprint

Osteoblasts
Fibroblasts
Prosthetics
Skin
Bone
Titanium
Histology
Biocompatibility
Encapsulation
Assays
Animals
Tissue

Keywords

  • autologous fibroblasts
  • direct skeletal attachment
  • osseointegration
  • prosthetic rehabilitation
  • skin- and bone-integrated pylon

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Two-stage implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts induced into osteoblast differentiation for direct skeletal attachment of limb prostheses. / Shevtsov, Maxim A.; Galibin, Oleg V.; Yudintceva, Nataliya M.; Blinova, Miralda I.; Pinaev, George P.; Ivanova, Anna A.; Savchenko, Olga N.; Suslov, Dmitriy N.; Potokin, Igor L.; Pitkin, Emil; Raykhtsaum, Grigory; Pitkin, Mark R.

In: Journal of Biomedical Materials Research - Part A, Vol. 102, No. 9, 01.01.2014, p. 3033-3048.

Research output: Contribution to journalArticle

Shevtsov, MA, Galibin, OV, Yudintceva, NM, Blinova, MI, Pinaev, GP, Ivanova, AA, Savchenko, ON, Suslov, DN, Potokin, IL, Pitkin, E, Raykhtsaum, G & Pitkin, MR 2014, 'Two-stage implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts induced into osteoblast differentiation for direct skeletal attachment of limb prostheses', Journal of Biomedical Materials Research - Part A, vol. 102, no. 9, pp. 3033-3048. https://doi.org/10.1002/jbm.a.34969
Shevtsov, Maxim A. ; Galibin, Oleg V. ; Yudintceva, Nataliya M. ; Blinova, Miralda I. ; Pinaev, George P. ; Ivanova, Anna A. ; Savchenko, Olga N. ; Suslov, Dmitriy N. ; Potokin, Igor L. ; Pitkin, Emil ; Raykhtsaum, Grigory ; Pitkin, Mark R. / Two-stage implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts induced into osteoblast differentiation for direct skeletal attachment of limb prostheses. In: Journal of Biomedical Materials Research - Part A. 2014 ; Vol. 102, No. 9. pp. 3033-3048.
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