Total nitrogen oxide following exercise testing reflects endothelial function and discriminates health status

Jason D. Allen, Frederick R. Cobb, William E. Kraus, Andrew J. Gow

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Nitric oxide (NO) bioavailability is important in vascular health, but unsuitable as a clinical measure due to biological oxidation. Total nitrogen oxides (NOx) are stable but background nitrate levels make it difficult to detect disease-based variation. We investigated the clinical discriminatory value of NOx as it relates to exercise capability (VO2peak) and brachial artery reactivity (BAR, an NO-dependent measure of endothelial health), in healthy (H), increased risk (RF), and known cardiovascular disease (CVD) subjects. BAR was measured using forearm occlusion/hyperemia stimulus. Subjects performed a maximal graded exercise test (GXT). Blood at rest, exercise termination, and 10 min into recovery was mixed equally with 0.1 M NaOH at 4°C, filtered, and stored at -70°C. NOx was measured by chemiluminescence. Seven of the RF group then exercise-trained for 6 months prior to retesting. The H group (n = 12) was younger, had higher VO2peak, HDL levels, and baseline NOx values than the RF (n = 15) and CVD (n = 10) groups. NOx increased from baseline to recovery in the H group only (75.85 ± 19.04 μM vs 97.76 ± 31.93 μM; P ≤ 0.01). BAR was greater in the H versus CVD group (7.24 ± 3.78% vs 2.59 ± 3.53%; P ≤ 0.01). The relation between VO2peak and NOx recovery was significant across groups (r = 0.71, P ≤ 0.01). Following training the RF subjects (n = 7) increased VO2peak (29.98 ± 6.74 to 33.08 ± 5.57 ml kg-1 min-1; P ≤ 0.05), BAR (3.19 ± 3.96 to 6.86 ± 4.81%; P ≤ 0.05), and recovery NOx (18.29 ± 6.46 to 66.48 + 21.11 μM; P ≤ 0.01). These findings suggest that plasma NOx following GXT discriminate cardiovascular disease status, are related to regional endothelial function, and respond favorably to exercise training.

Original languageEnglish
Pages (from-to)740-747
Number of pages8
JournalFree Radical Biology and Medicine
Volume41
Issue number5
DOIs
Publication statusPublished - 1 Sep 2006
Externally publishedYes

Fingerprint

Nitrogen Oxides
Health Status
Nitric Oxide
Health
Testing
Cardiovascular Diseases
Recovery
Exercise Test
Chemiluminescence
Brachial Artery
Hyperemia
Luminescence
Forearm
Nitrates
Biological Availability
Blood Vessels
Blood
Exercise
Plasmas
Oxidation

Keywords

  • Cardiovascular disease
  • Endothelium
  • Exercise
  • Nitric oxide
  • NO
  • VO

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Total nitrogen oxide following exercise testing reflects endothelial function and discriminates health status. / Allen, Jason D.; Cobb, Frederick R.; Kraus, William E.; Gow, Andrew J.

In: Free Radical Biology and Medicine, Vol. 41, No. 5, 01.09.2006, p. 740-747.

Research output: Contribution to journalArticle

Allen, Jason D. ; Cobb, Frederick R. ; Kraus, William E. ; Gow, Andrew J. / Total nitrogen oxide following exercise testing reflects endothelial function and discriminates health status. In: Free Radical Biology and Medicine. 2006 ; Vol. 41, No. 5. pp. 740-747.
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