The Study of ^99mTc-Aluminum Oxide Using for Sentinel Lymph Nodes Detection in Experiment

The purpose of the study was a comparative research of the possibility of using the radiopharmaceuticals ^99mTc-Al₂O₃ and ^99mTc-Nanocis for visualizing sentinel lymph nodes in the experiment. Measurement of the sizes of ^99mTc-Al₂O₃ and ^99mTc-Nanocis colloidal particles was performed in seven series of radiopharmaceuticals. Pharmacokinetics of ^99mTc-Al₂O₃ and ^99mTc-Nanocis was researched on 50 white male rats. The possibility of the use of ^99mTc-Al₂O₃ and ^99mTc-Nanocis for lymphoscintigraphy was studied in the experiments on 12 white male rats. Average dynamic diameter of the sol particle was 52-77 nm for ^99mTc-Al₂O₃ and 16.7-24.5 nm for ^99mTc-Nanocis. Radiopharmaceuticals accumulated in the inguinal lymph node - at 1 hour after administration the average uptake of ^99mTc-Al₂O₃ was 8.6% in it and the accumulation of ^99mTc-Nanocis was significantly lower - 1,8% (p <0,05). In all study point average uptake of ^99mTc-Al₂O₃ in the lymph node was significantly higher ^99mTc-Nanocis accumulation. The results dynamic scintigraphic studies in rats showed that ^99mTc-Al₂O₃ and ^99mTc-Nanocis actively accumulated into the lymphatic system. By using ^99mTc-Al₂O₃ inguinal lymph node was determined in 5 minutes after injection and clearly visualize in all the animals in the 15th minute, when the accumulation became more than 1% of the administered dose. Further observation indicated that the ^99mTc-Al₂O₃ accumulation reaches a plateau in a lymph node (average 10.5%) for 2 hour study and then its accumulation remains practically at the same level, slightly increasing to 12% at 24 hours. In the case of ^99mTc-Nanocis inguinal lymph node visualized in all animals for 15 minutes when it was accumulated on the average 1.03% of the administered dose. Plateau of ^99mTc-Nanocis accumulation in the lymph node (average 2.05%) occurred at 2 hour of the study and remained almost on the same level (in average 2.3%) to 24 hours. Thus, the experimental study of a new domestic radiopharmaceutical showed that the ^99mTc-Al₂O₃ several times actively accumulates in the lymph nodes as compared to imported analogue and its practical application will facilitate intraoperative identification of sentinel lymph nodes.