The study of platelet reaction on a-C:H:SiOxcoatings obtained via plasma enhanced chemical vapor deposition with bipolar bias voltage

M. O. Zhulkov, A. S. Grenadyorov, D. S. Korneev, H. A. Agaeva, A. M. Chernyavsky, I. A. Khlusov

Research output: Contribution to journalArticlepeer-review

Abstract

Aim. To study platelet adhesion to a-C:H:SiOxfilm on titanium in an in vitro experiment to evaluate its antithrombogenic potential. Materials and methods. Thin (less than 1 μm) a-C:H:SiOxfilms were deposited on VT-6 titanium plates with a size of 10 × 10 mm2and a thickness of 0.2 mm using a vacuum ion-plasma unit using pulsed bipolar bias. The surface roughness was evaluated according to GOST 2789-73 using an atomic force microscope. The test samples were cultured at 37 °C for 30 min in platelet-rich human blood plasma, prepared for scanning electron microscopy, after which the distribution density of blood plates adhering to the test coating was calculated. Results. With the same roughness index of the studied a-C:H:SiOxsamples, the film decreased 116 times (in comparison with untreated titanium) the platelet count per 1 mm2of the surface. Conclusion. The deposition of a-C:H:SiOxthin film on the surface of VT-6 titanium alloy by PACVD method using pulsed bipolar bias significantly reduces the distribution density of platelets in comparison with an untreated metal surface. In vitro data suggest a significant antithrombogenic potential of this type of coating on the surface of devices in contact with blood.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalBulletin of Siberian Medicine
Volume19
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • Atomic force microscopy
  • Carbonic surface modified by silicon oxides
  • Human platelet adhesion
  • In vitro
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Molecular Medicine

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