The Simultaneous Voltammetric Determination of Aflatoxins B₁ and M₁ on a Glassy-Carbon Electrode

For the first time, the possibility of using stripping voltammetry for the simultaneous determination of aflatoxins B₁ and M₁ on a glassy-carbon electrode has been shown. The influence of various factors (I_e, τ_e, w, and the nature of the background electrolyte) on the potential and magnitude of the oxidation current of mycotoxins has been estimated. Working conditions for voltammetric determination and reproducibility of analytical signals for two mycotoxins have been selected. The mutual influence of aflatoxins B₁ and M₁ on the value of analytical signals in their simultaneous presence has been studied. It has been found that, in the range of their detectable contents, the presence of aflatoxin B₁ reduces the analytical signal of aflatoxin M₁ by 45-50%, but the linearity of the calibration dependence is preserved. The content of aflatoxin M₁ in determination of aflatoxin B₁ does not exert a significant effect in the range of 10-15%. Based on the results obtained, a procedure has been proposed for determining the content of aflatoxins B₁ and M₁ in their joint presence in milk by voltammetry in the concentration ranges 2 × 10^-3 ÷ 2 × 10^-1 mg/dm³ and 2 × 10^-4 ÷ 2 × 10^-2 mg/dm³, respectively (Sr not more than 18%).