Abstract
A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage. (C) 2000 Published by Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 197-200 |
| Number of pages | 4 |
| Journal | Immunology Letters |
| Volume | 74 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Nov 2000 |
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Keywords
- Ballistic transfection
- Chimaeric antibody genes
- Immune response
- Immunoglobulin E
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes. / Rodin, Dmitry V.; Kolesnikov, Victor A.; Zelenina, Inessa A.; Zelenin, Alexander V.; Deyev, Sergey M.
In: Immunology Letters, Vol. 74, No. 3, 01.11.2000, p. 197-200.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes
AU - Rodin, Dmitry V.
AU - Kolesnikov, Victor A.
AU - Zelenina, Inessa A.
AU - Zelenin, Alexander V.
AU - Deyev, Sergey M.
PY - 2000/11/1
Y1 - 2000/11/1
N2 - A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage. (C) 2000 Published by Elsevier Science B.V.
AB - A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage. (C) 2000 Published by Elsevier Science B.V.
KW - Ballistic transfection
KW - Chimaeric antibody genes
KW - Immune response
KW - Immunoglobulin E
UR - http://www.scopus.com/inward/record.url?scp=0034330002&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034330002&partnerID=8YFLogxK
U2 - 10.1016/S0165-2478(00)00269-8
DO - 10.1016/S0165-2478(00)00269-8
M3 - Article
AN - SCOPUS:0034330002
VL - 74
SP - 197
EP - 200
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 3
ER -