The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes

Abstract

A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage. (C) 2000 Published by Elsevier Science B.V.

Original language	English
Pages (from-to)	197-200
Number of pages	4
Journal	Immunology Letters
Volume	74
Issue number	3
DOIs	https://doi.org/10.1016/S0165-2478(00)00269-8
Publication status	Published - 1 Nov 2000

Keywords

Ballistic transfection
Chimaeric antibody genes
Immune response
Immunoglobulin E

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0165-2478(00)00269-8

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title = "The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes",

abstract = "A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage. (C) 2000 Published by Elsevier Science B.V.",

keywords = "Ballistic transfection, Chimaeric antibody genes, Immune response, Immunoglobulin E",

author = "Rodin, {Dmitry V.} and Kolesnikov, {Victor A.} and Zelenina, {Inessa A.} and Zelenin, {Alexander V.} and Deyev, {Sergey M.}",

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T1 - The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes

AU - Rodin, Dmitry V.

AU - Kolesnikov, Victor A.

AU - Zelenina, Inessa A.

AU - Zelenin, Alexander V.

AU - Deyev, Sergey M.

PY - 2000/11/1

Y1 - 2000/11/1

N2 - A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage. (C) 2000 Published by Elsevier Science B.V.

AB - A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage. (C) 2000 Published by Elsevier Science B.V.

KW - Ballistic transfection

KW - Chimaeric antibody genes

KW - Immune response

KW - Immunoglobulin E

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