The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes

Dmitry V. Rodin, Victor A. Kolesnikov, Inessa A. Zelenina, Alexander V. Zelenin, Sergey M. Deyev

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage. (C) 2000 Published by Elsevier Science B.V.

    Original languageEnglish
    Pages (from-to)197-200
    Number of pages4
    JournalImmunology Letters
    Volume74
    Issue number3
    DOIs
    Publication statusPublished - 1 Nov 2000

    Fingerprint

    Transfection
    Antibodies
    Ear Cartilage
    Genes
    Immunoglobulin Genes
    Immunoglobulin E
    Foot
    Proteins
    Spleen
    Gene Expression
    Liver

    Keywords

    • Ballistic transfection
    • Chimaeric antibody genes
    • Immune response
    • Immunoglobulin E

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

    Cite this

    The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes. / Rodin, Dmitry V.; Kolesnikov, Victor A.; Zelenina, Inessa A.; Zelenin, Alexander V.; Deyev, Sergey M.

    In: Immunology Letters, Vol. 74, No. 3, 01.11.2000, p. 197-200.

    Research output: Contribution to journalArticle

    Rodin, Dmitry V. ; Kolesnikov, Victor A. ; Zelenina, Inessa A. ; Zelenin, Alexander V. ; Deyev, Sergey M. / The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes. In: Immunology Letters. 2000 ; Vol. 74, No. 3. pp. 197-200.
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