The luminol-amplified chemiluminescence of neutrophils and monocytes in patients with gastric cancer after intraoperative radiotherapy using radiosensitizer sanazole

Igor Schepetkin, Nadejda Cherdyntseva, Sergei Afanasjev, Sergei Antipov, Boris Zyryanov

Research output: Contribution to journalArticle

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Abstract

The effect of electron-beam intraoperative radiotherapy (IORT) with radiosensitizer sanazole (drug AK-2123) on the functional activity of neutrophils and monocytes in patients with gastric cancer was studied with the use of luminol-amplified chemiluminescence. Sanazole was administered intravenously in a dose of 1.2 g/m2, 30 rain before IORT. After resection of the tumor a single dose of 10 Gy was given to the field of regional lymph collectors and the bed of the removed tumor applying a small-size betatron MIB-6E. Cells were separated and chemiluminescence was estimated on the 1-st day before and on the 3-d day and 2-d week after IORT with sanazole. Insignificant effect on the zymosan- or phorbol-12-myristate-13-acetate (PMA)-stimulated chemiluminescence was observed in this group, although there was found a considerable increase of chemiluminescence of neutrophils in response to zymosan and PMA in 5 from 8 patients, and chemiluminescence of monocytes- in 3 from 8 patients. Correlation between individual indices of zymosan- and PMA-stimulated chemiluminescence of neutrophils before IORT with sanazole was not high, r=0.71, but it increased significantly to r=0.91 on the 3-d day and to r=0.96 on 2-d week after treatment. Correlation between individual indices of stimulated chemiluminescence of monocytes before IORT with sanazole was r=0.90, and increased to r=0.99 on the 3-d day and to r=0.96 on the 2-d week after treatment. So, it is indicative of a correcting effect of the combined treatment including IORT with sanazole administration on the functional activity of neutrophils and monocytes, and the absence of functional disturbances of these cells.

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalCancer Biotherapy and Radiopharmaceuticals
Volume14
Issue number5
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Luminol
Luminescence
Stomach Neoplasms
Monocytes
Neutrophils
Radiotherapy
Zymosan
Acetates
Particle Accelerators
Rain
Lymph
AK 2123
Neoplasms
Therapeutics
Electrons
Pharmaceutical Preparations

Keywords

  • Chemiluminescence
  • Gastric cancer
  • Intraoperative radiotherapy
  • Monocytes
  • Neutrophils
  • Radiosensitizer

ASJC Scopus subject areas

  • Cancer Research
  • Pharmacology
  • Oncology

Cite this

The luminol-amplified chemiluminescence of neutrophils and monocytes in patients with gastric cancer after intraoperative radiotherapy using radiosensitizer sanazole. / Schepetkin, Igor; Cherdyntseva, Nadejda; Afanasjev, Sergei; Antipov, Sergei; Zyryanov, Boris.

In: Cancer Biotherapy and Radiopharmaceuticals, Vol. 14, No. 5, 1999, p. 397-402.

Research output: Contribution to journalArticle

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AB - The effect of electron-beam intraoperative radiotherapy (IORT) with radiosensitizer sanazole (drug AK-2123) on the functional activity of neutrophils and monocytes in patients with gastric cancer was studied with the use of luminol-amplified chemiluminescence. Sanazole was administered intravenously in a dose of 1.2 g/m2, 30 rain before IORT. After resection of the tumor a single dose of 10 Gy was given to the field of regional lymph collectors and the bed of the removed tumor applying a small-size betatron MIB-6E. Cells were separated and chemiluminescence was estimated on the 1-st day before and on the 3-d day and 2-d week after IORT with sanazole. Insignificant effect on the zymosan- or phorbol-12-myristate-13-acetate (PMA)-stimulated chemiluminescence was observed in this group, although there was found a considerable increase of chemiluminescence of neutrophils in response to zymosan and PMA in 5 from 8 patients, and chemiluminescence of monocytes- in 3 from 8 patients. Correlation between individual indices of zymosan- and PMA-stimulated chemiluminescence of neutrophils before IORT with sanazole was not high, r=0.71, but it increased significantly to r=0.91 on the 3-d day and to r=0.96 on 2-d week after treatment. Correlation between individual indices of stimulated chemiluminescence of monocytes before IORT with sanazole was r=0.90, and increased to r=0.99 on the 3-d day and to r=0.96 on the 2-d week after treatment. So, it is indicative of a correcting effect of the combined treatment including IORT with sanazole administration on the functional activity of neutrophils and monocytes, and the absence of functional disturbances of these cells.

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