Abstract
Au nanoparticles (NPs) functionalized with L-cysteine (Cys) and cysteine-glycine (Cys- Gly) were synthetized. The AuNPs were prepared using sodium citrate as reducing agent. The influence of the molar concentrations of Cys and Cys-Gly, as well as the sodium citrate is studied on particle size and particle size distribution. TEM measurements revealed the formation of AuNPs with diameter in the range 5-35 nm which corresponds to nontoxic sizes [we should add a reference here, perhaps number one]. The optimal particle size for biomedical application along with narrow particle size distribution was observed for samples prepared with molar ratio of CAu:Ccitrate = 1:10. The results of UV-Vis spectroscopy revealed the interaction of the AuNPs with Cys and Gly-Cys demonstrated by a visible change in the absorption intensities of the plasmon peak located at 520 nm after AuNP functionalization and a slight shifting of this gold nanoparticles plasmon peak. Thus, any dielectric shell on surface of particles with more refraction index (and, correspondingly, dielectric function) can produce the particles with the red shift. Such effect of the surface shell with red-shift in the range of few nanometers observed for the AuNPs functionalized with Cys and Cys- Gly (Fig. 4) can be interpreted as thin or discontinuous layer of aminoacid molecules according to the data of optical spectra simulation. The optimum concentration of precursors was found for the suitable nontoxic AuNPs according to two main criteria. Firstly, particle size suitable to overcome blood brain barrier without being toxic (~20 nm) and nontoxic spherical shape. AuNPs obtained using 10 μM of citrate demonstrated the optimum narrow particle size distribution with 20 nm colloids preferentially formed. After particles functionalization with the aminoacid and peptide the particle size distribution did not change significantly. However, the number of particles with the size of 20 nm increases more than (add %) compare to the citrate method.
| Original language | English |
|---|---|
| Title of host publication | Advanced Materials Research |
| Pages | 94-105 |
| Number of pages | 12 |
| Volume | 872 |
| DOIs | |
| Publication status | Published - 2014 |
| Event | Russian-German Forum on Nanotechnology - Tomsk, Russian Federation Duration: 21 May 2013 → 24 May 2013 |
Publication series
| Name | Advanced Materials Research |
|---|---|
| Volume | 872 |
| ISSN (Print) | 10226680 |
Other
| Other | Russian-German Forum on Nanotechnology |
|---|---|
| Country | Russian Federation |
| City | Tomsk |
| Period | 21.5.13 → 24.5.13 |
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Keywords
- Cysteine
- Functionalization
- Glycine
- Gold nanoparticles
- Sodium citrate
ASJC Scopus subject areas
- Engineering(all)
Cite this
The influence of the peptide molar ratios on the functionalization of gold nanoparticles. / Ramirez-Camacho, Mayra C.; Tuzovskaya, Inga; Bogdanchikova, Nina; Pestryakov, Alexey; Susarrey-Arce, Arturo; Gurin, Valerii.
Advanced Materials Research. Vol. 872 2014. p. 94-105 (Advanced Materials Research; Vol. 872).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - The influence of the peptide molar ratios on the functionalization of gold nanoparticles
AU - Ramirez-Camacho, Mayra C.
AU - Tuzovskaya, Inga
AU - Bogdanchikova, Nina
AU - Pestryakov, Alexey
AU - Susarrey-Arce, Arturo
AU - Gurin, Valerii
PY - 2014
Y1 - 2014
N2 - Au nanoparticles (NPs) functionalized with L-cysteine (Cys) and cysteine-glycine (Cys- Gly) were synthetized. The AuNPs were prepared using sodium citrate as reducing agent. The influence of the molar concentrations of Cys and Cys-Gly, as well as the sodium citrate is studied on particle size and particle size distribution. TEM measurements revealed the formation of AuNPs with diameter in the range 5-35 nm which corresponds to nontoxic sizes [we should add a reference here, perhaps number one]. The optimal particle size for biomedical application along with narrow particle size distribution was observed for samples prepared with molar ratio of CAu:Ccitrate = 1:10. The results of UV-Vis spectroscopy revealed the interaction of the AuNPs with Cys and Gly-Cys demonstrated by a visible change in the absorption intensities of the plasmon peak located at 520 nm after AuNP functionalization and a slight shifting of this gold nanoparticles plasmon peak. Thus, any dielectric shell on surface of particles with more refraction index (and, correspondingly, dielectric function) can produce the particles with the red shift. Such effect of the surface shell with red-shift in the range of few nanometers observed for the AuNPs functionalized with Cys and Cys- Gly (Fig. 4) can be interpreted as thin or discontinuous layer of aminoacid molecules according to the data of optical spectra simulation. The optimum concentration of precursors was found for the suitable nontoxic AuNPs according to two main criteria. Firstly, particle size suitable to overcome blood brain barrier without being toxic (~20 nm) and nontoxic spherical shape. AuNPs obtained using 10 μM of citrate demonstrated the optimum narrow particle size distribution with 20 nm colloids preferentially formed. After particles functionalization with the aminoacid and peptide the particle size distribution did not change significantly. However, the number of particles with the size of 20 nm increases more than (add %) compare to the citrate method.
AB - Au nanoparticles (NPs) functionalized with L-cysteine (Cys) and cysteine-glycine (Cys- Gly) were synthetized. The AuNPs were prepared using sodium citrate as reducing agent. The influence of the molar concentrations of Cys and Cys-Gly, as well as the sodium citrate is studied on particle size and particle size distribution. TEM measurements revealed the formation of AuNPs with diameter in the range 5-35 nm which corresponds to nontoxic sizes [we should add a reference here, perhaps number one]. The optimal particle size for biomedical application along with narrow particle size distribution was observed for samples prepared with molar ratio of CAu:Ccitrate = 1:10. The results of UV-Vis spectroscopy revealed the interaction of the AuNPs with Cys and Gly-Cys demonstrated by a visible change in the absorption intensities of the plasmon peak located at 520 nm after AuNP functionalization and a slight shifting of this gold nanoparticles plasmon peak. Thus, any dielectric shell on surface of particles with more refraction index (and, correspondingly, dielectric function) can produce the particles with the red shift. Such effect of the surface shell with red-shift in the range of few nanometers observed for the AuNPs functionalized with Cys and Cys- Gly (Fig. 4) can be interpreted as thin or discontinuous layer of aminoacid molecules according to the data of optical spectra simulation. The optimum concentration of precursors was found for the suitable nontoxic AuNPs according to two main criteria. Firstly, particle size suitable to overcome blood brain barrier without being toxic (~20 nm) and nontoxic spherical shape. AuNPs obtained using 10 μM of citrate demonstrated the optimum narrow particle size distribution with 20 nm colloids preferentially formed. After particles functionalization with the aminoacid and peptide the particle size distribution did not change significantly. However, the number of particles with the size of 20 nm increases more than (add %) compare to the citrate method.
KW - Cysteine
KW - Functionalization
KW - Glycine
KW - Gold nanoparticles
KW - Sodium citrate
UR - http://www.scopus.com/inward/record.url?scp=84891891856&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891891856&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.872.94
DO - 10.4028/www.scientific.net/AMR.872.94
M3 - Conference contribution
SN - 9783037859667
VL - 872
T3 - Advanced Materials Research
SP - 94
EP - 105
BT - Advanced Materials Research
ER -