The influence of the peptide molar ratios on the functionalization of gold nanoparticles

Mayra C. Ramirez-Camacho, Inga Tuzovskaya, Nina Bogdanchikova, Alexey Pestryakov, Arturo Susarrey-Arce, Valerii Gurin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Au nanoparticles (NPs) functionalized with L-cysteine (Cys) and cysteine-glycine (Cys- Gly) were synthetized. The AuNPs were prepared using sodium citrate as reducing agent. The influence of the molar concentrations of Cys and Cys-Gly, as well as the sodium citrate is studied on particle size and particle size distribution. TEM measurements revealed the formation of AuNPs with diameter in the range 5-35 nm which corresponds to nontoxic sizes [we should add a reference here, perhaps number one]. The optimal particle size for biomedical application along with narrow particle size distribution was observed for samples prepared with molar ratio of CAu:Ccitrate = 1:10. The results of UV-Vis spectroscopy revealed the interaction of the AuNPs with Cys and Gly-Cys demonstrated by a visible change in the absorption intensities of the plasmon peak located at 520 nm after AuNP functionalization and a slight shifting of this gold nanoparticles plasmon peak. Thus, any dielectric shell on surface of particles with more refraction index (and, correspondingly, dielectric function) can produce the particles with the red shift. Such effect of the surface shell with red-shift in the range of few nanometers observed for the AuNPs functionalized with Cys and Cys- Gly (Fig. 4) can be interpreted as thin or discontinuous layer of aminoacid molecules according to the data of optical spectra simulation. The optimum concentration of precursors was found for the suitable nontoxic AuNPs according to two main criteria. Firstly, particle size suitable to overcome blood brain barrier without being toxic (~20 nm) and nontoxic spherical shape. AuNPs obtained using 10 μM of citrate demonstrated the optimum narrow particle size distribution with 20 nm colloids preferentially formed. After particles functionalization with the aminoacid and peptide the particle size distribution did not change significantly. However, the number of particles with the size of 20 nm increases more than (add %) compare to the citrate method.

Original languageEnglish
Title of host publicationAdvanced Materials Research
Pages94-105
Number of pages12
Volume872
DOIs
Publication statusPublished - 2014
EventRussian-German Forum on Nanotechnology - Tomsk, Russian Federation
Duration: 21 May 201324 May 2013

Publication series

NameAdvanced Materials Research
Volume872
ISSN (Print)10226680

Other

OtherRussian-German Forum on Nanotechnology
CountryRussian Federation
CityTomsk
Period21.5.1324.5.13

Fingerprint

Particle size analysis
Peptides
Gold
Nanoparticles
Particle size
Amino acids
Sodium
Reducing agents
Ultraviolet spectroscopy
Refraction
Colloids
Particles (particulate matter)
Transmission electron microscopy
Molecules

Keywords

  • Cysteine
  • Functionalization
  • Glycine
  • Gold nanoparticles
  • Sodium citrate

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Ramirez-Camacho, M. C., Tuzovskaya, I., Bogdanchikova, N., Pestryakov, A., Susarrey-Arce, A., & Gurin, V. (2014). The influence of the peptide molar ratios on the functionalization of gold nanoparticles. In Advanced Materials Research (Vol. 872, pp. 94-105). (Advanced Materials Research; Vol. 872). https://doi.org/10.4028/www.scientific.net/AMR.872.94

The influence of the peptide molar ratios on the functionalization of gold nanoparticles. / Ramirez-Camacho, Mayra C.; Tuzovskaya, Inga; Bogdanchikova, Nina; Pestryakov, Alexey; Susarrey-Arce, Arturo; Gurin, Valerii.

Advanced Materials Research. Vol. 872 2014. p. 94-105 (Advanced Materials Research; Vol. 872).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ramirez-Camacho, MC, Tuzovskaya, I, Bogdanchikova, N, Pestryakov, A, Susarrey-Arce, A & Gurin, V 2014, The influence of the peptide molar ratios on the functionalization of gold nanoparticles. in Advanced Materials Research. vol. 872, Advanced Materials Research, vol. 872, pp. 94-105, Russian-German Forum on Nanotechnology, Tomsk, Russian Federation, 21.5.13. https://doi.org/10.4028/www.scientific.net/AMR.872.94
Ramirez-Camacho MC, Tuzovskaya I, Bogdanchikova N, Pestryakov A, Susarrey-Arce A, Gurin V. The influence of the peptide molar ratios on the functionalization of gold nanoparticles. In Advanced Materials Research. Vol. 872. 2014. p. 94-105. (Advanced Materials Research). https://doi.org/10.4028/www.scientific.net/AMR.872.94
Ramirez-Camacho, Mayra C. ; Tuzovskaya, Inga ; Bogdanchikova, Nina ; Pestryakov, Alexey ; Susarrey-Arce, Arturo ; Gurin, Valerii. / The influence of the peptide molar ratios on the functionalization of gold nanoparticles. Advanced Materials Research. Vol. 872 2014. pp. 94-105 (Advanced Materials Research).
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abstract = "Au nanoparticles (NPs) functionalized with L-cysteine (Cys) and cysteine-glycine (Cys- Gly) were synthetized. The AuNPs were prepared using sodium citrate as reducing agent. The influence of the molar concentrations of Cys and Cys-Gly, as well as the sodium citrate is studied on particle size and particle size distribution. TEM measurements revealed the formation of AuNPs with diameter in the range 5-35 nm which corresponds to nontoxic sizes [we should add a reference here, perhaps number one]. The optimal particle size for biomedical application along with narrow particle size distribution was observed for samples prepared with molar ratio of CAu:Ccitrate = 1:10. The results of UV-Vis spectroscopy revealed the interaction of the AuNPs with Cys and Gly-Cys demonstrated by a visible change in the absorption intensities of the plasmon peak located at 520 nm after AuNP functionalization and a slight shifting of this gold nanoparticles plasmon peak. Thus, any dielectric shell on surface of particles with more refraction index (and, correspondingly, dielectric function) can produce the particles with the red shift. Such effect of the surface shell with red-shift in the range of few nanometers observed for the AuNPs functionalized with Cys and Cys- Gly (Fig. 4) can be interpreted as thin or discontinuous layer of aminoacid molecules according to the data of optical spectra simulation. The optimum concentration of precursors was found for the suitable nontoxic AuNPs according to two main criteria. Firstly, particle size suitable to overcome blood brain barrier without being toxic (~20 nm) and nontoxic spherical shape. AuNPs obtained using 10 μM of citrate demonstrated the optimum narrow particle size distribution with 20 nm colloids preferentially formed. After particles functionalization with the aminoacid and peptide the particle size distribution did not change significantly. However, the number of particles with the size of 20 nm increases more than (add {\%}) compare to the citrate method.",
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