The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity

E. V. Mitroshina, T. A. Mishchenko, M. V. Vedunova, A. V. Yudintsev, A. N. Generalova, A. V. Nechaev, S. M. Deyev, I. V. Mukhina, Andrei V. Zvyagin

Research output: Contribution to journalArticle

Abstract

The aim of the investigation was to study the effect of upconversion nanoparticles (UCNPs) with different surface coatings on viability and functional neural network activity of primary hippocampal cultures. Materials and Methods. The UCNPs with three surface coating modifications (tetramethylammonium hydroxide (TMAH), polymaleic acid octadecene and polyethyleneimine (PEI)) were synthesized by a solvothermal technique with further hydrophilization. Primary hippocampal cultures, obtained from C57BL/6 mice embryos (E18), were incubated with tested UCNPs in the concentration of 0.8 and 8 mg/ml during 72 h. The cell viability detection, evaluation of morphological changes by immunocytochemical staining as well as the UCNPs influence on the main parameters of the functional neural network calcium activity and on the endocytosis processes were carried out. Results. Our studies revealed that UCNPs caused a dose-dependent cytotoxic effect on primary hippocampal cultures, wherein the severity of this effect directly related to the type of UCNPs surface coating. The greatest cytotoxicity was identified for UCNP-PEI, and the least — for UCNP-TMAH. UCNPs toxicity is manifested in significant morphological changes of neural networks and in the increase the number of dead cells (p<0.05) in primary hippocampal cultures. Moreover, a significant decrease (p<0.05) in the main parameters of spontaneous functional calcium activity was shown. Conclusion. A comprehensive investigation of the nanoparticles effects on primary hippocampal cultures showed that all tested UCNPs have the strong toxic effect to the nervous system cells.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalSovremennye Tehnologii v Medicine
Volume8
Issue number4
DOIs
Publication statusPublished - 2016
Externally publishedYes

Fingerprint

Nanoparticles
Coatings
Polyethyleneimine
Neural networks
Calcium
Poisons
Neurology
Cytotoxicity
Endocytosis
Inbred C57BL Mouse
Nervous System
Toxicity
Cell Survival
Embryonic Structures
Cell Count
Cells
Staining and Labeling
Acids

Keywords

  • Functional network activity
  • Neural network
  • Particle toxicology
  • Primary hippocampal cultures
  • Upconversion nanoparticles

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mitroshina, E. V., Mishchenko, T. A., Vedunova, M. V., Yudintsev, A. V., Generalova, A. N., Nechaev, A. V., ... Zvyagin, A. V. (2016). The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity. Sovremennye Tehnologii v Medicine, 8(4), 133-141. https://doi.org/10.17691/stm2016.8.4.18

The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity. / Mitroshina, E. V.; Mishchenko, T. A.; Vedunova, M. V.; Yudintsev, A. V.; Generalova, A. N.; Nechaev, A. V.; Deyev, S. M.; Mukhina, I. V.; Zvyagin, Andrei V.

In: Sovremennye Tehnologii v Medicine, Vol. 8, No. 4, 2016, p. 133-141.

Research output: Contribution to journalArticle

Mitroshina, EV, Mishchenko, TA, Vedunova, MV, Yudintsev, AV, Generalova, AN, Nechaev, AV, Deyev, SM, Mukhina, IV & Zvyagin, AV 2016, 'The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity', Sovremennye Tehnologii v Medicine, vol. 8, no. 4, pp. 133-141. https://doi.org/10.17691/stm2016.8.4.18
Mitroshina EV, Mishchenko TA, Vedunova MV, Yudintsev AV, Generalova AN, Nechaev AV et al. The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity. Sovremennye Tehnologii v Medicine. 2016;8(4):133-141. https://doi.org/10.17691/stm2016.8.4.18
Mitroshina, E. V. ; Mishchenko, T. A. ; Vedunova, M. V. ; Yudintsev, A. V. ; Generalova, A. N. ; Nechaev, A. V. ; Deyev, S. M. ; Mukhina, I. V. ; Zvyagin, Andrei V. / The influence of different types of upconversion nanoparticles surface coatings on neurotoxicity. In: Sovremennye Tehnologii v Medicine. 2016 ; Vol. 8, No. 4. pp. 133-141.
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AU - Generalova, A. N.

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