Abstract
The cDNA of a previously uncharacterized gene fet5 (factor of eukaryotic transcription, clone no. 5) of the fission yeast Schizosaccharomyces pombe was cloned by the heterospecific complementation of a conditional mutant of Saccharomyces cerevisiae defective in the function of the RNA polymerases I-III common subunit ABC10β. The gene encodes a new factor of eukaryotic transcription, Fet5, the first member of a superfamily of proteins for which the area of functioning is determined. The Fet5-superfamily consists of three distinct families of proteins highly evolutionarily conserved and widely spread among eukaryotes. Features of the Fet5 amino acid sequence suggest that it belongs to ATP/GTP-binding proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 237 |
| Number of pages | 1 |
| Journal | Bioorganicheskaya Khimiya |
| Volume | 23 |
| Issue number | 3 |
| Publication status | Published - Mar 1997 |
| Externally published | Yes |
Keywords
- Common subunit ABC10β
- Fet5 gene
- Heterospecific suppressor
- Multicopy extragenic suppressor
- New protein superfamily
- Purine nucleotides binding proteins
- RNA polymerases I-III
- Schizosaccharomyces pombe
- Transcription factor
ASJC Scopus subject areas
- Medicine(all)