The First Member of a Novel Family of Protein Factors of Eukaryotic Transcription Discovered by the Heterospecific Complementation

G. V. Shpakovski, E. N. Lebedenko

Research output: Contribution to journalArticlepeer-review

Abstract

The cDNA of a previously uncharacterized gene fet5 (factor of eukaryotic transcription, clone no. 5) of the fission yeast Schizosaccharomyces pombe was cloned by the heterospecific complementation of a conditional mutant of Saccharomyces cerevisiae defective in the function of the RNA polymerases I-III common subunit ABC10β. The gene encodes a new factor of eukaryotic transcription, Fet5, the first member of a superfamily of proteins for which the area of functioning is determined. The Fet5-superfamily consists of three distinct families of proteins highly evolutionarily conserved and widely spread among eukaryotes. Features of the Fet5 amino acid sequence suggest that it belongs to ATP/GTP-binding proteins.

Original languageEnglish
Pages (from-to)237
Number of pages1
JournalBioorganicheskaya Khimiya
Volume23
Issue number3
Publication statusPublished - Mar 1997
Externally publishedYes

Keywords

  • Common subunit ABC10β
  • Fet5 gene
  • Heterospecific suppressor
  • Multicopy extragenic suppressor
  • New protein superfamily
  • Purine nucleotides binding proteins
  • RNA polymerases I-III
  • Schizosaccharomyces pombe
  • Transcription factor

ASJC Scopus subject areas

  • Medicine(all)

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