The effect of the targeted recombinant toxin DARPin-PE40 on the dynamics of HER2-positive tumor growth

E. A. Sokolova, G. M. Proshkina, O. M. Kutova, I. V. Balalaeva, S. M. Deyev

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The development of targeted toxins based on non-immunoglobulin targeting molecules appears to be one of the most advanced approaches in the targeted therapy of malignant tumors with a high expression of the HER2 receptor. Earlier, we showed that the targeted toxin DARPin-PE40 consisting of the HER2-specific non-immunoglobulin polypeptide (the targeting module) and a fragment of Pseudomonas exotoxin A (the toxic module) exhibits an antitumor effect in vivo against the HER2-positive adenocarcinoma xenograft. In this work, an in-depth analysis of the effect of DARPin-PE40 on the growth dynamics of experimental xenograft tumors was carried out. DARPin-PE40 was shown to inhibit tumor growth at a dose of 25 and 50 μg/animal and to cause tumor node reduction at a dose of 80 μg/animal, followed by growth resumption at the end of therapy. An evaluation of the tumor growth dynamics revealed statistically significant differences in tumor volume in mice in the experimental groups compared to the control group. The results testify to the potential of using the created targeted toxin as an agent for the targeted therapy of HER2-overexpressing tumors.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalActa Naturae
Volume9
Issue number3
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • HER2 receptor
  • Non-immunoglobulin module DARPin
  • Pseudomonas aeruginosa exotoxin A
  • Targeted therapy

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

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