The effect of porosity on cell ingrowth into accurately defined, laser-made, polylactide-based 3D scaffolds

Paulius Danilevicius, Leoni Georgiadi, Christopher J. Pateman, Frederik Claeyssens, Maria Chatzinikolaidou, Maria Farsari

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

The aim of this study is to demonstrate the accuracy required for the investigation of the role of solid scaffolds' porosity in cell proliferation. We therefore present a qualitative investigation into the effect of porosity on MC3T3-E1 pre-osteoblastic cell ingrowth of three-dimensional (3D) scaffolds fabricated by direct femtosecond laser writing. The material we used is a purpose made photosensitive pre-polymer based on polylactide. We designed and fabricated complex, geometry-controlled 3D scaffolds with pore sizes ranging from 25 to 110 μm, representing porosities 70%, 82%, 86%, and 90%. The 70% porosity scaffolds did not support cell growth initially and in the long term. For the other porosities, we found a strong adhesion of the pre-osteoblastic cells from the first hours after seeding and a remarkable proliferation increase after 3 weeks and up to 8 weeks. The 86% porosity scaffolds exhibited a higher efficiency compared to 82% and 90%. In addition, bulk material degradation studies showed that the employed, highly-acrylated polylactide is degradable. These findings support the potential use of the proposed material and the scaffold fabrication technique in bone tissue engineering.

Original languageEnglish
Pages (from-to)2-10
Number of pages9
JournalApplied Surface Science
Volume336
DOIs
Publication statusPublished - 1 May 2015
Externally publishedYes

Keywords

  • 3D scaffolds
  • Polylactide
  • Porosity
  • Tissue engineering
  • Tissue regeneration

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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