Abstract
The method of luminol-dependent chemiluminescence (CL) was used to study the influence of gamma- and fast electrons-irradiation on secretion of reactive oxygen species of neutrophils in enrich suspension and in presence of all blood components. In vitro gamma-irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous CL and activated stimulated by phorbol-12-myristate-13-acetate (PMA) (100 nM) in the period 30-60 min after radiation. The time of maximum PMA-stimulated CL amplitude was decreased with the dose range from 2(5) to 25(30) Gy for gamma- and fast electrons-irradiation. The addition of radiosensitizer AK-2123 (0.05% or 2.2 mM) depressed the early PMA-stimulated CL response on gamma- and fast electrons-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils.
| Translated title of the contribution | The effect of ionizing radiation and radiosensitizing agent AK-2123 on luminol-dependent chemiluminescence of human neutrophils |
|---|---|
| Original language | Russian |
| Pages (from-to) | 601-608 |
| Number of pages | 8 |
| Journal | Radiatsionnaya Biologiya Radioekologiya |
| Volume | 38 |
| Issue number | 4 |
| Publication status | Published - Jul 1998 |
| Externally published | Yes |
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Ecology
- Radiology Nuclear Medicine and imaging
- Radiation