The method of luminol-dependent chemiluminescence (CL) was used to study the influence of gamma- and fast electrons-irradiation on secretion of reactive oxygen species of neutrophils in enrich suspension and in presence of all blood components. In vitro gamma-irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous CL and activated stimulated by phorbol-12-myristate-13-acetate (PMA) (100 nM) in the period 30-60 min after radiation. The time of maximum PMA-stimulated CL amplitude was decreased with the dose range from 2(5) to 25(30) Gy for gamma- and fast electrons-irradiation. The addition of radiosensitizer AK-2123 (0.05% or 2.2 mM) depressed the early PMA-stimulated CL response on gamma- and fast electrons-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils.
|Translated title of the contribution||The effect of ionizing radiation and radiosensitizing agent AK-2123 on luminol-dependent chemiluminescence of human neutrophils|
|Number of pages||8|
|Journal||Radiatsionnaya Biologiya Radioekologiya|
|Publication status||Published - Jul 1998|
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Radiology Nuclear Medicine and imaging