Vliianie ioniziruiushchego izlucheniia i radiosensibilizatora AK-2123 na liuminolzavisimuiu khemiliuminestsentsiiu neitrofilov cheloveka.

Translated title of the contribution: The effect of ionizing radiation and radiosensitizing agent AK-2123 on luminol-dependent chemiluminescence of human neutrophils

Research output: Contribution to journalArticle

Abstract

The method of luminol-dependent chemiluminescence (CL) was used to study the influence of gamma- and fast electrons-irradiation on secretion of reactive oxygen species of neutrophils in enrich suspension and in presence of all blood components. In vitro gamma-irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous CL and activated stimulated by phorbol-12-myristate-13-acetate (PMA) (100 nM) in the period 30-60 min after radiation. The time of maximum PMA-stimulated CL amplitude was decreased with the dose range from 2(5) to 25(30) Gy for gamma- and fast electrons-irradiation. The addition of radiosensitizer AK-2123 (0.05% or 2.2 mM) depressed the early PMA-stimulated CL response on gamma- and fast electrons-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils.

Original languageRussian
Pages (from-to)601-608
Number of pages8
JournalRadiatsionnaya Biologiya Radioekologiya
Volume38
Issue number4
Publication statusPublished - Jul 1998
Externally publishedYes

Fingerprint

Radiation-Sensitizing Agents
neutrophils
Luminol
Chemiluminescence
Ionizing radiation
chemiluminescence
Luminescence
Ionizing Radiation
ionizing radiation
Electron irradiation
irradiation
Neutrophils
electron irradiation
acetates
acetate
Acetates
Electrons
electron
Suspensions
secretions

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Ecology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

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title = "Vliianie ioniziruiushchego izlucheniia i radiosensibilizatora AK-2123 na liuminolzavisimuiu khemiliuminestsentsiiu neitrofilov cheloveka.",
abstract = "The method of luminol-dependent chemiluminescence (CL) was used to study the influence of gamma- and fast electrons-irradiation on secretion of reactive oxygen species of neutrophils in enrich suspension and in presence of all blood components. In vitro gamma-irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous CL and activated stimulated by phorbol-12-myristate-13-acetate (PMA) (100 nM) in the period 30-60 min after radiation. The time of maximum PMA-stimulated CL amplitude was decreased with the dose range from 2(5) to 25(30) Gy for gamma- and fast electrons-irradiation. The addition of radiosensitizer AK-2123 (0.05{\%} or 2.2 mM) depressed the early PMA-stimulated CL response on gamma- and fast electrons-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils.",
author = "Shchepetkin, {I. A.}",
year = "1998",
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AU - Shchepetkin, I. A.

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N2 - The method of luminol-dependent chemiluminescence (CL) was used to study the influence of gamma- and fast electrons-irradiation on secretion of reactive oxygen species of neutrophils in enrich suspension and in presence of all blood components. In vitro gamma-irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous CL and activated stimulated by phorbol-12-myristate-13-acetate (PMA) (100 nM) in the period 30-60 min after radiation. The time of maximum PMA-stimulated CL amplitude was decreased with the dose range from 2(5) to 25(30) Gy for gamma- and fast electrons-irradiation. The addition of radiosensitizer AK-2123 (0.05% or 2.2 mM) depressed the early PMA-stimulated CL response on gamma- and fast electrons-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils.

AB - The method of luminol-dependent chemiluminescence (CL) was used to study the influence of gamma- and fast electrons-irradiation on secretion of reactive oxygen species of neutrophils in enrich suspension and in presence of all blood components. In vitro gamma-irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous CL and activated stimulated by phorbol-12-myristate-13-acetate (PMA) (100 nM) in the period 30-60 min after radiation. The time of maximum PMA-stimulated CL amplitude was decreased with the dose range from 2(5) to 25(30) Gy for gamma- and fast electrons-irradiation. The addition of radiosensitizer AK-2123 (0.05% or 2.2 mM) depressed the early PMA-stimulated CL response on gamma- and fast electrons-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils.

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