Abstract
The results of the investigation of the proliferation effect on a mesenchymal stem cell (MSC) culture and the estimates on cytotoxicity of surfaces of titanium nickelide specimens prepared using special mechanical and electrochemical methods and characterized by different morphology and roughness are presented. The specimens of the alloy based on titanium nickelide are shown not to exert any toxic action on the MSCs of rats. When cultivated in the presence of the tested materials or being on their surfaces, MSCs preserved their viability, adhesive and morphological properties, and the ability for in vitro proliferation. This was confirmed by the following methods: cell counting in a Goryaev chamber, MTT, flow cytometry, and light and fluorescent microscopy. It was revealed that the proliferation processes are weakly pronounced on the surface of TH1(B) specimens whose C10-11 class roughness was achieved by multistage mechanical grinding to "high luster" and subsequent electrolytic grinding. On the contrary, the C7 class roughness of the TH1(A) specimen surfaces achieved by chemical etching and subsequent electrolytic grinding is more optimal for MSC proliferation.
| Original language | English |
|---|---|
| Pages (from-to) | 135-144 |
| Number of pages | 10 |
| Journal | Inorganic Materials: Applied Research |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Apr 2012 |
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Keywords
- Biocompatibility
- Cytotoxicity
- Mesenchymal stem cells of rats
- Proliferation
- Roughness
- Titanium nickelide
ASJC Scopus subject areas
- Materials Science(all)
- Engineering(all)
Cite this
The effect of chemical composition and roughness of titanium nickelide surface on proliferative properties of mesenchymal stem cells. / Lotkov, A. I.; Psakh'E, S. G.; Meisner, L. L.; Matveeva, V. A.; Artem'Eva, L. V.; Meisner, S. N.; Matveev, A. L.
In: Inorganic Materials: Applied Research, Vol. 3, No. 2, 01.04.2012, p. 135-144.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of chemical composition and roughness of titanium nickelide surface on proliferative properties of mesenchymal stem cells
AU - Lotkov, A. I.
AU - Psakh'E, S. G.
AU - Meisner, L. L.
AU - Matveeva, V. A.
AU - Artem'Eva, L. V.
AU - Meisner, S. N.
AU - Matveev, A. L.
PY - 2012/4/1
Y1 - 2012/4/1
N2 - The results of the investigation of the proliferation effect on a mesenchymal stem cell (MSC) culture and the estimates on cytotoxicity of surfaces of titanium nickelide specimens prepared using special mechanical and electrochemical methods and characterized by different morphology and roughness are presented. The specimens of the alloy based on titanium nickelide are shown not to exert any toxic action on the MSCs of rats. When cultivated in the presence of the tested materials or being on their surfaces, MSCs preserved their viability, adhesive and morphological properties, and the ability for in vitro proliferation. This was confirmed by the following methods: cell counting in a Goryaev chamber, MTT, flow cytometry, and light and fluorescent microscopy. It was revealed that the proliferation processes are weakly pronounced on the surface of TH1(B) specimens whose C10-11 class roughness was achieved by multistage mechanical grinding to "high luster" and subsequent electrolytic grinding. On the contrary, the C7 class roughness of the TH1(A) specimen surfaces achieved by chemical etching and subsequent electrolytic grinding is more optimal for MSC proliferation.
AB - The results of the investigation of the proliferation effect on a mesenchymal stem cell (MSC) culture and the estimates on cytotoxicity of surfaces of titanium nickelide specimens prepared using special mechanical and electrochemical methods and characterized by different morphology and roughness are presented. The specimens of the alloy based on titanium nickelide are shown not to exert any toxic action on the MSCs of rats. When cultivated in the presence of the tested materials or being on their surfaces, MSCs preserved their viability, adhesive and morphological properties, and the ability for in vitro proliferation. This was confirmed by the following methods: cell counting in a Goryaev chamber, MTT, flow cytometry, and light and fluorescent microscopy. It was revealed that the proliferation processes are weakly pronounced on the surface of TH1(B) specimens whose C10-11 class roughness was achieved by multistage mechanical grinding to "high luster" and subsequent electrolytic grinding. On the contrary, the C7 class roughness of the TH1(A) specimen surfaces achieved by chemical etching and subsequent electrolytic grinding is more optimal for MSC proliferation.
KW - Biocompatibility
KW - Cytotoxicity
KW - Mesenchymal stem cells of rats
KW - Proliferation
KW - Roughness
KW - Titanium nickelide
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UR - http://www.scopus.com/inward/citedby.url?scp=84864538429&partnerID=8YFLogxK
U2 - 10.1134/S2075113312020116
DO - 10.1134/S2075113312020116
M3 - Article
VL - 3
SP - 135
EP - 144
JO - Inorganic Materials: Applied Research
JF - Inorganic Materials: Applied Research
SN - 2075-1133
IS - 2
ER -