The effect of chemical composition and roughness of titanium nickelide surface on proliferative properties of mesenchymal stem cells

A. I. Lotkov, S. G. Psakh'E, L. L. Meisner, V. A. Matveeva, L. V. Artem'Eva, S. N. Meisner, A. L. Matveev

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The results of the investigation of the proliferation effect on a mesenchymal stem cell (MSC) culture and the estimates on cytotoxicity of surfaces of titanium nickelide specimens prepared using special mechanical and electrochemical methods and characterized by different morphology and roughness are presented. The specimens of the alloy based on titanium nickelide are shown not to exert any toxic action on the MSCs of rats. When cultivated in the presence of the tested materials or being on their surfaces, MSCs preserved their viability, adhesive and morphological properties, and the ability for in vitro proliferation. This was confirmed by the following methods: cell counting in a Goryaev chamber, MTT, flow cytometry, and light and fluorescent microscopy. It was revealed that the proliferation processes are weakly pronounced on the surface of TH1(B) specimens whose C10-11 class roughness was achieved by multistage mechanical grinding to "high luster" and subsequent electrolytic grinding. On the contrary, the C7 class roughness of the TH1(A) specimen surfaces achieved by chemical etching and subsequent electrolytic grinding is more optimal for MSC proliferation.

Original languageEnglish
Pages (from-to)135-144
Number of pages10
JournalInorganic Materials: Applied Research
Volume3
Issue number2
DOIs
Publication statusPublished - 1 Apr 2012

Fingerprint

Stem cells
Titanium
Surface roughness
Chemical analysis
Toxic Actions
Flow cytometry
Cell proliferation
Cytotoxicity
Cell culture
Rats
Etching
Adhesives
Microscopic examination
titanium nickelide

Keywords

  • Biocompatibility
  • Cytotoxicity
  • Mesenchymal stem cells of rats
  • Proliferation
  • Roughness
  • Titanium nickelide

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)

Cite this

Lotkov, A. I., Psakh'E, S. G., Meisner, L. L., Matveeva, V. A., Artem'Eva, L. V., Meisner, S. N., & Matveev, A. L. (2012). The effect of chemical composition and roughness of titanium nickelide surface on proliferative properties of mesenchymal stem cells. Inorganic Materials: Applied Research, 3(2), 135-144. https://doi.org/10.1134/S2075113312020116

The effect of chemical composition and roughness of titanium nickelide surface on proliferative properties of mesenchymal stem cells. / Lotkov, A. I.; Psakh'E, S. G.; Meisner, L. L.; Matveeva, V. A.; Artem'Eva, L. V.; Meisner, S. N.; Matveev, A. L.

In: Inorganic Materials: Applied Research, Vol. 3, No. 2, 01.04.2012, p. 135-144.

Research output: Contribution to journalArticle

Lotkov, A. I. ; Psakh'E, S. G. ; Meisner, L. L. ; Matveeva, V. A. ; Artem'Eva, L. V. ; Meisner, S. N. ; Matveev, A. L. / The effect of chemical composition and roughness of titanium nickelide surface on proliferative properties of mesenchymal stem cells. In: Inorganic Materials: Applied Research. 2012 ; Vol. 3, No. 2. pp. 135-144.
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