The effect of chemical composition and roughness of titanium nickelide surface on proliferative properties of mesenchymal stem cells

The results of the investigation of the proliferation effect on a mesenchymal stem cell (MSC) culture and the estimates on cytotoxicity of surfaces of titanium nickelide specimens prepared using special mechanical and electrochemical methods and characterized by different morphology and roughness are presented. The specimens of the alloy based on titanium nickelide are shown not to exert any toxic action on the MSCs of rats. When cultivated in the presence of the tested materials or being on their surfaces, MSCs preserved their viability, adhesive and morphological properties, and the ability for in vitro proliferation. This was confirmed by the following methods: cell counting in a Goryaev chamber, MTT, flow cytometry, and light and fluorescent microscopy. It was revealed that the proliferation processes are weakly pronounced on the surface of TH1(B) specimens whose C10-11 class roughness was achieved by multistage mechanical grinding to "high luster" and subsequent electrolytic grinding. On the contrary, the C7 class roughness of the TH1(A) specimen surfaces achieved by chemical etching and subsequent electrolytic grinding is more optimal for MSC proliferation.