Testing the in vitro performance of hydroxyapatite coated magnesium (AZ91D) and titanium concerning cell adhesion and osteogenic differentiation

Claudia Kleinhans, Gabriele Vacun, Roman Surmenev, Maria Surmeneva, Petra Juliane Kluger

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In the current study the in vitro outcome of a degradable magnesium alloy (AZ91D) and standard titanium modified by nanostructured-hydroxyapatite (n-HA) coatings concerning cell adhesion and osteogenic differentiation was investigated by direct cell culture. The n-HA modification was prepared via radio-frequency magnetron sputtering deposition and proven by field emission scanning electron microscopy and X-ray powder diffraction patterns revealing a homogenous surface coating. Human mesenchymal stem cell (hMSCs) adhesion was examined after one and 14 days displaying an enhanced initial cell adhesion on the n-HA modified samples. The osteogenic lineage commitment of the cells was determined by alkaline phosphatase (ALP) quantification. On day one n-HA coated AZ91D exhibited a comparable ALP expression to standard tissue culture polystyrene samples. However, after 14 days solely little DNA and ALP amounts were measurable on n-HA coated AZ91D due to the lack of adherent cells. Titanium displayed excellent cell adhesion properties and ALP was detectable after 14 days. An increased pH of the culture was measured for AZ91D as well as for n-HA coated AZ91D. We conclude that n-HA modification improves initial cell attachment on AZ91D within the first 24 h. However, the effect does not persist for 14 days in in vitro conditions.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalBioNanoMaterials
Volume16
Issue number1
DOIs
Publication statusPublished - 1 Mar 2015

Fingerprint

Cell adhesion
Durapatite
Titanium
Hydroxyapatite
Magnesium
Phosphatases
Testing
Alkaline Phosphatase
Coatings
Tissue culture
Polystyrenes
Magnesium alloys
Stem cells
Cell culture
Field emission
Magnetron sputtering
X ray powder diffraction
Diffraction patterns
DNA
Scanning electron microscopy

Keywords

  • cell adhesion
  • direct cell test
  • human mesenchymal stem cells
  • in vitro testing
  • magnesium alloys
  • nanostructured-hydroxyapatite coating
  • osteoinductivity

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

Cite this

Testing the in vitro performance of hydroxyapatite coated magnesium (AZ91D) and titanium concerning cell adhesion and osteogenic differentiation. / Kleinhans, Claudia; Vacun, Gabriele; Surmenev, Roman; Surmeneva, Maria; Kluger, Petra Juliane.

In: BioNanoMaterials, Vol. 16, No. 1, 01.03.2015, p. 41-50.

Research output: Contribution to journalArticle

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