Temperature-responsive PLLA/PNIPAM nanofibers for switchable release

Roman Elashnikov, Petr Slepička, Silvie Rimpelova, Pavel Ulbrich, Vaclav Švorčík, Oleksiy Lyutakov

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Smart antimicrobial materials with on-demand drug release are highly desired for biomedical applications. Herein, we report about temperature-responsive poly(N-isopropylacrylamide) (PNIPAM) nanospheres doped with crystal violet (CV) and incorporated into the poly-L-lactide (PLLA) nanofibers. The nanofibers were prepared by electrospinning, using different initial polymers ratios. The morphology of the nanofibers and polymers distribution in the nanofibers were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The interaction between PNIPAM and PLLA in the nanofibers was studied by Fourier transform infrared spectroscopy (FTIR) and its effect on the PNIPAM phase transition was also investigated. It was shown that by the changing of the environmental temperature across the lower critical solution temperature (LCST) of PNIPAM, the switchable wettability and controlled CV release can be achieved. The temperature-dependent release kinetics of CV from polymer nanofibers was investigated by ultraviolet-visible spectroscopy (UV–Vis). The temperature-responsive release of antibacterial CV was also tested for triggering of antibacterial activity, which was examined on Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli). Thus, the proposed material is promising value for controllable drug-release.

Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalMaterials Science and Engineering C
Volume72
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Fingerprint

Nanofibers
Gentian Violet
crystals
Polymers
Crystals
polymers
drugs
Temperature
smart materials
temperature
staphylococcus
Escherichia
wettability
ambient temperature
Nanospheres
infrared spectroscopy
Ultraviolet visible spectroscopy
Electrospinning
atomic force microscopy
Pharmaceutical Preparations

Keywords

  • Antibacterial
  • Electrospinning
  • Nanofibers
  • Polymer blends
  • Responsive release
  • Stimuli-responsive
  • Switchable wettability

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Temperature-responsive PLLA/PNIPAM nanofibers for switchable release. / Elashnikov, Roman; Slepička, Petr; Rimpelova, Silvie; Ulbrich, Pavel; Švorčík, Vaclav; Lyutakov, Oleksiy.

In: Materials Science and Engineering C, Vol. 72, 01.03.2017, p. 293-300.

Research output: Contribution to journalArticle

Elashnikov, Roman ; Slepička, Petr ; Rimpelova, Silvie ; Ulbrich, Pavel ; Švorčík, Vaclav ; Lyutakov, Oleksiy. / Temperature-responsive PLLA/PNIPAM nanofibers for switchable release. In: Materials Science and Engineering C. 2017 ; Vol. 72. pp. 293-300.
@article{6eceab112aa340bfb86f2bc538b1f766,
title = "Temperature-responsive PLLA/PNIPAM nanofibers for switchable release",
abstract = "Smart antimicrobial materials with on-demand drug release are highly desired for biomedical applications. Herein, we report about temperature-responsive poly(N-isopropylacrylamide) (PNIPAM) nanospheres doped with crystal violet (CV) and incorporated into the poly-L-lactide (PLLA) nanofibers. The nanofibers were prepared by electrospinning, using different initial polymers ratios. The morphology of the nanofibers and polymers distribution in the nanofibers were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The interaction between PNIPAM and PLLA in the nanofibers was studied by Fourier transform infrared spectroscopy (FTIR) and its effect on the PNIPAM phase transition was also investigated. It was shown that by the changing of the environmental temperature across the lower critical solution temperature (LCST) of PNIPAM, the switchable wettability and controlled CV release can be achieved. The temperature-dependent release kinetics of CV from polymer nanofibers was investigated by ultraviolet-visible spectroscopy (UV–Vis). The temperature-responsive release of antibacterial CV was also tested for triggering of antibacterial activity, which was examined on Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli). Thus, the proposed material is promising value for controllable drug-release.",
keywords = "Antibacterial, Electrospinning, Nanofibers, Polymer blends, Responsive release, Stimuli-responsive, Switchable wettability",
author = "Roman Elashnikov and Petr Slepička and Silvie Rimpelova and Pavel Ulbrich and Vaclav Švorč{\'i}k and Oleksiy Lyutakov",
year = "2017",
month = "3",
day = "1",
doi = "10.1016/j.msec.2016.11.028",
language = "English",
volume = "72",
pages = "293--300",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Temperature-responsive PLLA/PNIPAM nanofibers for switchable release

AU - Elashnikov, Roman

AU - Slepička, Petr

AU - Rimpelova, Silvie

AU - Ulbrich, Pavel

AU - Švorčík, Vaclav

AU - Lyutakov, Oleksiy

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Smart antimicrobial materials with on-demand drug release are highly desired for biomedical applications. Herein, we report about temperature-responsive poly(N-isopropylacrylamide) (PNIPAM) nanospheres doped with crystal violet (CV) and incorporated into the poly-L-lactide (PLLA) nanofibers. The nanofibers were prepared by electrospinning, using different initial polymers ratios. The morphology of the nanofibers and polymers distribution in the nanofibers were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The interaction between PNIPAM and PLLA in the nanofibers was studied by Fourier transform infrared spectroscopy (FTIR) and its effect on the PNIPAM phase transition was also investigated. It was shown that by the changing of the environmental temperature across the lower critical solution temperature (LCST) of PNIPAM, the switchable wettability and controlled CV release can be achieved. The temperature-dependent release kinetics of CV from polymer nanofibers was investigated by ultraviolet-visible spectroscopy (UV–Vis). The temperature-responsive release of antibacterial CV was also tested for triggering of antibacterial activity, which was examined on Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli). Thus, the proposed material is promising value for controllable drug-release.

AB - Smart antimicrobial materials with on-demand drug release are highly desired for biomedical applications. Herein, we report about temperature-responsive poly(N-isopropylacrylamide) (PNIPAM) nanospheres doped with crystal violet (CV) and incorporated into the poly-L-lactide (PLLA) nanofibers. The nanofibers were prepared by electrospinning, using different initial polymers ratios. The morphology of the nanofibers and polymers distribution in the nanofibers were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The interaction between PNIPAM and PLLA in the nanofibers was studied by Fourier transform infrared spectroscopy (FTIR) and its effect on the PNIPAM phase transition was also investigated. It was shown that by the changing of the environmental temperature across the lower critical solution temperature (LCST) of PNIPAM, the switchable wettability and controlled CV release can be achieved. The temperature-dependent release kinetics of CV from polymer nanofibers was investigated by ultraviolet-visible spectroscopy (UV–Vis). The temperature-responsive release of antibacterial CV was also tested for triggering of antibacterial activity, which was examined on Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli). Thus, the proposed material is promising value for controllable drug-release.

KW - Antibacterial

KW - Electrospinning

KW - Nanofibers

KW - Polymer blends

KW - Responsive release

KW - Stimuli-responsive

KW - Switchable wettability

UR - http://www.scopus.com/inward/record.url?scp=84998704736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84998704736&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2016.11.028

DO - 10.1016/j.msec.2016.11.028

M3 - Article

C2 - 28024589

AN - SCOPUS:84998704736

VL - 72

SP - 293

EP - 300

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -