Targeted delivery of quantum dots to the HER2-expressing tumor using recombinant antibodies

I. V. Balalaeva, T. A. Zdobnova, E. A. Sokolova, S. M. Deyev

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Targeted delivery of semiconductor quantum dots (QDs) to tumors overexpressing HER2 cancer marker has been demonstrated on immunocompromised mice bearing human breast cancer xenografts. To obtain targeted QDs complexes we applied the approach based on the use of protein adaptor system, RNAase barnase and its inhibitor barstar. Specific binding to target cancer marker was achieved through bivalent fusion protein containing two fragments of 4D5scFv recombinant antibody and a fragment of barnase. QDs were conjugated to barstar, and final assembly of targeted complexes was obtained through non-covalent specific interaction of barstar, attached to QD, and barnase, that is part of the recombinant targeting protein. The efficient delivery of QDs to HER2-expressing tumor demonstrates the possibilities and prospects of the approach for targeted delivery of nanoparticles to cancer cells in vivo as the way to improve the efficiency of diagnosis and promote development of therapies based on the use of nanoparticles.

Original languageEnglish
Pages (from-to)536-542
Number of pages7
JournalRussian Journal of Bioorganic Chemistry
Volume41
Issue number5
DOIs
Publication statusPublished - 29 Sep 2015
Externally publishedYes

Keywords

  • cancer marker HER2
  • D5scFv
  • fluorescence
  • quantum dots

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry

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