Systemic administration of platelet-activating factor in rat reduces specific pulmonary uptake of circulating monoclonal antibody to angiotensin-converting enzyme

Elena Nikolaevna Atochina, Holger H. Hiemisch, Vladimir R. Muzykantov, Sergei M. Danilov

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Abstract

The biodistribution of radiolabeled mouse monoclonal antibody (MoAb) to angiotensin-converting enzyme (ACE) and control, nonimmune mouse IgG in platelet activating factor (PAF)-treated rats was studied. The blood level of both preparations was slightly decreased (90% of the control) in PAF-treated rats. Specific pulmonary accumulation of anti-ACE MoAb was reduced to 50% of control in contrast to a doubling in nonspecific pulmonary uptake of non-immune IgG. The changes in anti-ACE MoAb biodistribution were lung-specific and were accompanied by decrease in the pulmonary ACE activity (to 60% of control) and increase in serum ACE activity (to 170% of control). Thus anti-ACE MoAb reveals PAF-induced changes in the status of the pulmonary ACE and therefore can be used for the studies of pathology of the pulmonary endothelium.

Original languageEnglish
Pages (from-to)349-358
Number of pages10
JournalLung
Volume170
Issue number6
DOIs
Publication statusPublished - 1992
Externally publishedYes

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Keywords

  • Angiotensin-converting enzyme
  • Endothelium
  • Monoclonal antibody
  • Platelet-activating factor

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology

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