Sintez gena zrelogo interleǐkina 1alpha cheloveka putem amplifikatsii in vitro dupleksa mRNK-kDNK.

Translated title of the contribution: Synthesis of the mature human interleukin-1(alpha) gene by amplification of an mRNA-cDNA duplex in vitro

E. N. Lebedenko, O. V. Pultalov, I. A. Berlin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Mixture of polyadenylated mRNAs from human monocytes has been subjected to the reverse transcription specifically initiated from the mRNA encoding interleukin 1 alpha by a synthetic polynucleotide complementary to the mRNA's coding 3'-end to yield the corresponding mRNA-cDNA duplex. Under conditions of the polymerase chain reaction, with the above polynucleotide as a downstream primer and an upstream primer corresponding to the beginning of the mature interleukin 1 alpha (AA 113-271) gene, the mRNA-cDNA duplex yielded the desired gene, whose structure was proved by the restriction and sequence analyses. The gene, containing translation initiation and termination triplets, can be used for producing interleukin 1 alpha in various expression systems and as a probe in studies of the lymphokine's biosynthesis. This method of the gene synthesis does not need construction and analysis of cDNA libraries nor synthesis of double-stranded DNA, and can, in principle, make use of the total (non-fractionated) cellular RNA.

Translated title of the contributionSynthesis of the mature human interleukin-1(alpha) gene by amplification of an mRNA-cDNA duplex in vitro
Original languageRussian
Pages (from-to)1570-1573
Number of pages4
JournalBioorganicheskaia khimiia
Volume16
Issue number11
Publication statusPublished - Nov 1990

ASJC Scopus subject areas

  • Medicine(all)

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