Synthesis of N-succinimidyl 4-[76Br]bromobenzoate and its use in conjugation labelling of macromolecules

Ulrika Yngve, Elisabeth Hedberg, Anna Lövqvist, Vladimir Tolmachev, Bengt Långström

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The 76Br radiobromination of proteins and modified oligonucleotides by the use of N-succinimidyl 4-[76Br]bromobenzoate are described. The labelled N-succinimidyl bromobenzoate was synthesised from the corresponding trimethyltin compound, N-succinimidyl 4-trimethylstannylbenzoate, in 45-60% isolated radio-chemical yield with a specific radioactivity of 20-200 GBq μmol-1. The use of N-succinimidyl 4-[76Br]bromobenzoate in conjugation with the proteins human serum albumin and chromogranin and with 5′-hexylamino-modified oligodeoxy-nucleotides (ODN) and its phosphorothioate analogues (S-ODN), is described. The oligonucleotide sequence chosen for this work is an antisense sequence for the m-RNA encoding for chromogranin in rat.

Original languageEnglish
Pages (from-to)508-512
Number of pages5
JournalActa Chemica Scandinavica
Volume53
Issue number7
DOIs
Publication statusPublished - Jul 1999

ASJC Scopus subject areas

  • Chemical Engineering(all)

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