Synthesis of Magnetic Nanoparticles Stabilized by Magnetite-Binding Protein for Targeted Delivery to Cancer Cells

Abstract

A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnase*barstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnase*barstar pair mediates the interaction between nanoparticles and the component for modification. The efficiency of this method was confirmed in the synthesis of magnetite nanoparticles recognizing the HER2/neu tumor marker and in the selective labeling of HER2/neu with these nanoparticles on the surface of cancer cells.

Original language	English
Pages (from-to)	198-200
Number of pages	3
Journal	Doklady Biochemistry and Biophysics
Volume	481
Issue number	1
DOIs	https://doi.org/10.1134/S1607672918040051
Publication status	Published - 1 Jul 2018
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Chemistry(all)

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Kotelnikova, P. A., Shipunova, V. O., Aghayeva, U. F., Stremovskiy, O. A., Nikitin, M. P., Novikov, I. A., Schulga, A. A., Deyev, S. M., & Petrov, R. V. (2018). Synthesis of Magnetic Nanoparticles Stabilized by Magnetite-Binding Protein for Targeted Delivery to Cancer Cells. Doklady Biochemistry and Biophysics, 481(1), 198-200. https://doi.org/10.1134/S1607672918040051

Synthesis of Magnetic Nanoparticles Stabilized by Magnetite-Binding Protein for Targeted Delivery to Cancer Cells. / Kotelnikova, P. A.; Shipunova, V. O.; Aghayeva, U. F.; Stremovskiy, O. A.; Nikitin, M. P.; Novikov, I. A.; Schulga, A. A.; Deyev, S. M.; Petrov, R. V.

In: Doklady Biochemistry and Biophysics, Vol. 481, No. 1, 01.07.2018, p. 198-200.

Research output: Contribution to journal › Article › peer-review

@article{d09c934fe8f84a85bc6d086c5d7e4310,

title = "Synthesis of Magnetic Nanoparticles Stabilized by Magnetite-Binding Protein for Targeted Delivery to Cancer Cells",

abstract = "A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnase*barstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnase*barstar pair mediates the interaction between nanoparticles and the component for modification. The efficiency of this method was confirmed in the synthesis of magnetite nanoparticles recognizing the HER2/neu tumor marker and in the selective labeling of HER2/neu with these nanoparticles on the surface of cancer cells.",

author = "Kotelnikova, {P. A.} and Shipunova, {V. O.} and Aghayeva, {U. F.} and Stremovskiy, {O. A.} and Nikitin, {M. P.} and Novikov, {I. A.} and Schulga, {A. A.} and Deyev, {S. M.} and Petrov, {R. V.}",

year = "2018",

month = jul,

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doi = "10.1134/S1607672918040051",

language = "English",

volume = "481",

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journal = "Doklady Biochemistry and Biophysics",

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AU - Kotelnikova, P. A.

AU - Shipunova, V. O.

AU - Aghayeva, U. F.

AU - Stremovskiy, O. A.

AU - Nikitin, M. P.

AU - Novikov, I. A.

AU - Schulga, A. A.

AU - Deyev, S. M.

AU - Petrov, R. V.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnase*barstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnase*barstar pair mediates the interaction between nanoparticles and the component for modification. The efficiency of this method was confirmed in the synthesis of magnetite nanoparticles recognizing the HER2/neu tumor marker and in the selective labeling of HER2/neu with these nanoparticles on the surface of cancer cells.

AB - A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnase*barstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnase*barstar pair mediates the interaction between nanoparticles and the component for modification. The efficiency of this method was confirmed in the synthesis of magnetite nanoparticles recognizing the HER2/neu tumor marker and in the selective labeling of HER2/neu with these nanoparticles on the surface of cancer cells.

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Synthesis of Magnetic Nanoparticles Stabilized by Magnetite-Binding Protein for Targeted Delivery to Cancer Cells

Abstract

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