Abstract
Genes for the heavy and light chains of a chimeric (mouse/human) IgE were introduced into various mouse tissues using ballistic transfection in vivo. The full-size chimeric IgE content in the blood plasma was evaluated in an immunochemical test. The IgE content in blood after its transfection of the cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. After a second transfection at the 114th day, neither chimeric IgE nor antibodies to it were found in the mouse blood. These data can be employed in selecting conditions for genetic immunization.
| Original language | English |
|---|---|
| Pages (from-to) | 12-17 |
| Number of pages | 6 |
| Journal | Molecular Biology |
| Volume | 34 |
| Issue number | 1 |
| Publication status | Published - Jan 2000 |
| Externally published | Yes |
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Keywords
- Ballistic transfection
- Chimeric antibody genes
- DNA immunization
- Immune response
- Immunoglobulin E
ASJC Scopus subject areas
- Structural Biology
- Biophysics
Cite this
Synthesis in vivo of chimeric IgE after ballistic transfection of various mouse tissues with immunoglobulin genes. / Rodin, D. V.; Kolesnikov, V. A.; Serdyuk, O. V.; Zelenina, I. A.; Zelenin, A. V.; Deyev, S. M.
In: Molecular Biology, Vol. 34, No. 1, 01.2000, p. 12-17.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis in vivo of chimeric IgE after ballistic transfection of various mouse tissues with immunoglobulin genes
AU - Rodin, D. V.
AU - Kolesnikov, V. A.
AU - Serdyuk, O. V.
AU - Zelenina, I. A.
AU - Zelenin, A. V.
AU - Deyev, S. M.
PY - 2000/1
Y1 - 2000/1
N2 - Genes for the heavy and light chains of a chimeric (mouse/human) IgE were introduced into various mouse tissues using ballistic transfection in vivo. The full-size chimeric IgE content in the blood plasma was evaluated in an immunochemical test. The IgE content in blood after its transfection of the cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. After a second transfection at the 114th day, neither chimeric IgE nor antibodies to it were found in the mouse blood. These data can be employed in selecting conditions for genetic immunization.
AB - Genes for the heavy and light chains of a chimeric (mouse/human) IgE were introduced into various mouse tissues using ballistic transfection in vivo. The full-size chimeric IgE content in the blood plasma was evaluated in an immunochemical test. The IgE content in blood after its transfection of the cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. After a second transfection at the 114th day, neither chimeric IgE nor antibodies to it were found in the mouse blood. These data can be employed in selecting conditions for genetic immunization.
KW - Ballistic transfection
KW - Chimeric antibody genes
KW - DNA immunization
KW - Immune response
KW - Immunoglobulin E
UR - http://www.scopus.com/inward/record.url?scp=0033632920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033632920&partnerID=8YFLogxK
M3 - Article
VL - 34
SP - 12
EP - 17
JO - Molecular Biology
JF - Molecular Biology
SN - 0026-8933
IS - 1
ER -