Synthesis in vivo of chimeric IgE after ballistic transfection of various mouse tissues with immunoglobulin genes

Abstract

Genes for the heavy and light chains of a chimeric (mouse/human) IgE were introduced into various mouse tissues using ballistic transfection in vivo. The full-size chimeric IgE content in the blood plasma was evaluated in an immunochemical test. The IgE content in blood after its transfection of the cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. After a second transfection at the 114th day, neither chimeric IgE nor antibodies to it were found in the mouse blood. These data can be employed in selecting conditions for genetic immunization.

Original language	English
Pages (from-to)	12-17
Number of pages	6
Journal	Molecular Biology
Volume	34
Issue number	1
Publication status	Published - Jan 2000
Externally published	Yes

Keywords

Ballistic transfection
Chimeric antibody genes
DNA immunization
Immune response
Immunoglobulin E

ASJC Scopus subject areas

Structural Biology
Biophysics

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title = "Synthesis in vivo of chimeric IgE after ballistic transfection of various mouse tissues with immunoglobulin genes",

abstract = "Genes for the heavy and light chains of a chimeric (mouse/human) IgE were introduced into various mouse tissues using ballistic transfection in vivo. The full-size chimeric IgE content in the blood plasma was evaluated in an immunochemical test. The IgE content in blood after its transfection of the cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. After a second transfection at the 114th day, neither chimeric IgE nor antibodies to it were found in the mouse blood. These data can be employed in selecting conditions for genetic immunization.",

keywords = "Ballistic transfection, Chimeric antibody genes, DNA immunization, Immune response, Immunoglobulin E",

author = "Rodin, {D. V.} and Kolesnikov, {V. A.} and Serdyuk, {O. V.} and Zelenina, {I. A.} and Zelenin, {A. V.} and Deyev, {S. M.}",

year = "2000",

month = jan,

language = "English",

volume = "34",

pages = "12--17",

journal = "Molecular Biology",

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T1 - Synthesis in vivo of chimeric IgE after ballistic transfection of various mouse tissues with immunoglobulin genes

AU - Rodin, D. V.

AU - Kolesnikov, V. A.

AU - Serdyuk, O. V.

AU - Zelenina, I. A.

AU - Zelenin, A. V.

AU - Deyev, S. M.

PY - 2000/1

Y1 - 2000/1

N2 - Genes for the heavy and light chains of a chimeric (mouse/human) IgE were introduced into various mouse tissues using ballistic transfection in vivo. The full-size chimeric IgE content in the blood plasma was evaluated in an immunochemical test. The IgE content in blood after its transfection of the cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. After a second transfection at the 114th day, neither chimeric IgE nor antibodies to it were found in the mouse blood. These data can be employed in selecting conditions for genetic immunization.

AB - Genes for the heavy and light chains of a chimeric (mouse/human) IgE were introduced into various mouse tissues using ballistic transfection in vivo. The full-size chimeric IgE content in the blood plasma was evaluated in an immunochemical test. The IgE content in blood after its transfection of the cartilage was found to be several times lower than after transfection of the liver, spleen, or foot pad. At the same time, the content of antibodies to the xenogenous determinants of IgE was essentially independent of the tissue type. After a second transfection at the 114th day, neither chimeric IgE nor antibodies to it were found in the mouse blood. These data can be employed in selecting conditions for genetic immunization.

KW - Ballistic transfection

KW - Chimeric antibody genes

KW - DNA immunization

KW - Immune response

KW - Immunoglobulin E

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