Synthesis, Characterization, and Selective Delivery of DARPin-Gold Nanoparticle Conjugates to Cancer Cells

Sergey Deyev, Galina Proshkina, Anastasiya Ryabova, Francesco Tavanti, Maria Cristina Menziani, Gennady Eidelshtein, Gavriel Avishai, Alexander Kotlyar

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We demonstrate that the designed ankyrin repeat protein (DARPin)-9-29, which specifically targets human epidermal growth factor receptor 2 (HER 2), binds tightly to gold nanoparticles (GNPs). Binding of the protein strongly increases the colloidal stability of the particles. The results of experimental analysis and molecular dynamics simulations show that approximately 35 DARPin-9-29 molecules are bound to the surface of a 5 nm GNP and that the binding does not involve the receptor-binding domain of the protein. The confocal fluorescent microscopy studies show that the DARPin-coated GNP conjugate specifically interacts with the surface of human cancer cells overexpressing epidermal growth factor receptor 2 (HER2) and enters the cells by endocytosis. The high stability under physiological conditions and high affinity to the receptors overexpressed by cancer cells make conjugates of plasmonic gold nanostructures with DARPin molecules promising candidates for cancer therapy.

Original languageEnglish
Pages (from-to)2569-2574
Number of pages6
JournalBioconjugate Chemistry
Volume28
Issue number10
DOIs
Publication statusPublished - 18 Oct 2017

Fingerprint

Ankyrin Repeat
Gold
Nanoparticles
Cells
Proteins
Neoplasms
Carrier Proteins
Nanostructures
Molecular Dynamics Simulation
Endocytosis
Epidermal Growth Factor Receptor
Molecules
Confocal Microscopy
Molecular dynamics
Microscopic examination
Computer simulation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Deyev, S., Proshkina, G., Ryabova, A., Tavanti, F., Menziani, M. C., Eidelshtein, G., ... Kotlyar, A. (2017). Synthesis, Characterization, and Selective Delivery of DARPin-Gold Nanoparticle Conjugates to Cancer Cells. Bioconjugate Chemistry, 28(10), 2569-2574. https://doi.org/10.1021/acs.bioconjchem.7b00410

Synthesis, Characterization, and Selective Delivery of DARPin-Gold Nanoparticle Conjugates to Cancer Cells. / Deyev, Sergey; Proshkina, Galina; Ryabova, Anastasiya; Tavanti, Francesco; Menziani, Maria Cristina; Eidelshtein, Gennady; Avishai, Gavriel; Kotlyar, Alexander.

In: Bioconjugate Chemistry, Vol. 28, No. 10, 18.10.2017, p. 2569-2574.

Research output: Contribution to journalArticle

Deyev, S, Proshkina, G, Ryabova, A, Tavanti, F, Menziani, MC, Eidelshtein, G, Avishai, G & Kotlyar, A 2017, 'Synthesis, Characterization, and Selective Delivery of DARPin-Gold Nanoparticle Conjugates to Cancer Cells', Bioconjugate Chemistry, vol. 28, no. 10, pp. 2569-2574. https://doi.org/10.1021/acs.bioconjchem.7b00410
Deyev, Sergey ; Proshkina, Galina ; Ryabova, Anastasiya ; Tavanti, Francesco ; Menziani, Maria Cristina ; Eidelshtein, Gennady ; Avishai, Gavriel ; Kotlyar, Alexander. / Synthesis, Characterization, and Selective Delivery of DARPin-Gold Nanoparticle Conjugates to Cancer Cells. In: Bioconjugate Chemistry. 2017 ; Vol. 28, No. 10. pp. 2569-2574.
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