Synthesis and modification of genes through artificial splicing by directed ligation (ASDL).

E. N. Lebedenko, K. R. Birikh, O. V. Plutalov, YuA Berlin YuA

Research output: Contribution to journalArticlepeer-review

Abstract

An approach to the directed genetic recombination in vitro mediated by synthetic oligodeoxynucleotides and polymerase chain reaction (PCR) is devised, which allows the joining, in a predetermined chemical-enzymatic way, of a series of DNA segments to give a precisely spliced polynucleotide sequence (Artificial Splicing by Directed Ligation, ASDL). The approach can thus lead to the totally processed eukaryotic genes using genomic DNA, with no mRNA needed. This approach has been used for the synthesis of artificial genes of interleukin-1 alpha and, in combination with PCR on the mRNA-cDNA duplex as template, of interleukin-1 receptor antagonist and their analogues, as well as for the modified genes.

Original languageEnglish
Pages (from-to)215-216
Number of pages2
JournalNucleic acids symposium series
Issue number24
Publication statusPublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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