Surfactant Protein-D, a Mediator of Innate Lung Immunity, Alters the Products of Nitric Oxide Metabolism

Elena Nikolaevna Atochina, Michael F. Beers, Samuel Hawgood, Francis Poulain, Christiana Davis, Trevor Fusaro, Andrew J. Gow

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Surfactant protein (SP)-D, a 43-kD multifunctional collagen-like lectin, is synthesized and secreted by the airway epithelium. SP-D knockout (SP-D [-/-]) mice exhibit an increase in the number and size of airway macrophages, peribronchiolar inflammation, increases in metalloproteinase activity, and development of emphysema. Nitric oxide (NO) is involved in a variety of signaling processes, and because altered NO metabolism has been observed in inflammation, we hypothesized that alterations in its metabolism would underlie the proinflammatory state observed in SP-D deficiency. Examination of the bronchial alveolar lavage (BAL) from SP-D (-/-) mice reveals a significant increase in protein and phospholipid content and total cell count. NO production and inducible NO synthase expression were increased in the BAL; however, there was a decline in 5-nitrosothiol (SNO) content in the BAL and a loss of SNO immunoreactivity within the tissue. This decline in SNO was accompanied by an increase in nitrotyrosine staining. We conclude that inflammation that occurs in SP-D deficiency results in an increase in NO production and a shift in the chemistry and targets of NO. We speculate that the proinflammatory response due to SP-D deficiency results, in part, from a disruption of NO-mediated signaling within the innate immune system.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume30
Issue number3
DOIs
Publication statusPublished - Mar 2004
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein D
Innate Immunity
Metabolism
Nitric Oxide
Lung
Protein Deficiency
Bronchoalveolar Lavage
Inflammation
Macrophages
Immune system
Emphysema
Metalloproteases
Nitric Oxide Synthase Type II
Lectins
Immune System
Phospholipids
Collagen
Epithelium
Cell Count
Tissue

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Surfactant Protein-D, a Mediator of Innate Lung Immunity, Alters the Products of Nitric Oxide Metabolism. / Atochina, Elena Nikolaevna; Beers, Michael F.; Hawgood, Samuel; Poulain, Francis; Davis, Christiana; Fusaro, Trevor; Gow, Andrew J.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 30, No. 3, 03.2004, p. 271-279.

Research output: Contribution to journalArticle

Atochina, Elena Nikolaevna ; Beers, Michael F. ; Hawgood, Samuel ; Poulain, Francis ; Davis, Christiana ; Fusaro, Trevor ; Gow, Andrew J. / Surfactant Protein-D, a Mediator of Innate Lung Immunity, Alters the Products of Nitric Oxide Metabolism. In: American Journal of Respiratory Cell and Molecular Biology. 2004 ; Vol. 30, No. 3. pp. 271-279.
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