Surface modification of biomaterials based on high-molecular polylactic acid and their effect on inflammatory reactions of primary human monocyte-derived macrophages: Perspective for personalized therapy

Ksenia Sergeevna Stankevich, Alexandru Gudima, Victor D. Filimonov, Harald Klüter, Evgeniya M. Mamontova, Sergei I. Tverdokhlebov, Julia Kzhyshkowska

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Polylactic acid (PLA) based implants can cause inflammatory complications. Macrophages are key innate immune cells that control inflammation. To provide higher biocompatibility of PLA-based implants with local innate immune cells their surface properties have to be improved. In our study surface modification technique for high-molecular PLA (MW = 1,646,600 g/mol) based biomaterials was originally developed and successfully applied. Optimal modification conditions were determined. Treatment of PLA films with toluene/ethanol = 3/7 mixture for 10 min with subsequent exposure in 0.001 M brilliant green dye (BGD) solution allows to entrap approximately 10- 9 mol/cm2 model biomolecules. The modified PLA film surface was characterized by optical microscopy, SERS, FT-IR, UV and TG/DTA/DSC analysis. Tensile strain of modified films was determined as well. The effect of PLA films modified with BGD on the inflammatory reactions of primary human monocyte-derived macrophages was investigated. We developed in vitro test-system by differentiating primary monocyte-derived macrophages on a coating material. Type 1 and type 2 inflammatory cytokines (TNFα, CCL18) secretion and histological biomarkers (CD206, stabilin-1) expression were analyzed by ELISA and confocal microscopy respectively. BGD-modified materials have improved thermal stability and good mechanical properties. However, BGD modifications induced additional donor-specific inflammatory reactions and suppressed tolerogenic phenotype of macrophages. Therefore, our test-system successfully demonstrated specific immunomodulatory effects of original and modified PLA-based biomaterials, and can be further applied for the examination of improved coatings for implants and identification of patient-specific reactions to implants.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalMaterials Science and Engineering C
Volume51
DOIs
Publication statusPublished - 1 Jun 2015

Keywords

  • Biocompatibility
  • Cytokines
  • Macrophages
  • Monocytes
  • Polylactic acid
  • Surface modification

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

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