Surface modification of biomaterials based on high-molecular polylactic acid and their effect on inflammatory reactions of primary human monocyte-derived macrophages

Perspective for personalized therapy

Ksenia Sergeevna Stankevich, Alexandru Gudima, Victor D. Filimonov, Harald Klüter, Evgeniya M. Mamontova, Sergei I. Tverdokhlebov, Julia Kzhyshkowska

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Polylactic acid (PLA) based implants can cause inflammatory complications. Macrophages are key innate immune cells that control inflammation. To provide higher biocompatibility of PLA-based implants with local innate immune cells their surface properties have to be improved. In our study surface modification technique for high-molecular PLA (MW = 1,646,600 g/mol) based biomaterials was originally developed and successfully applied. Optimal modification conditions were determined. Treatment of PLA films with toluene/ethanol = 3/7 mixture for 10 min with subsequent exposure in 0.001 M brilliant green dye (BGD) solution allows to entrap approximately 10- 9 mol/cm2 model biomolecules. The modified PLA film surface was characterized by optical microscopy, SERS, FT-IR, UV and TG/DTA/DSC analysis. Tensile strain of modified films was determined as well. The effect of PLA films modified with BGD on the inflammatory reactions of primary human monocyte-derived macrophages was investigated. We developed in vitro test-system by differentiating primary monocyte-derived macrophages on a coating material. Type 1 and type 2 inflammatory cytokines (TNFα, CCL18) secretion and histological biomarkers (CD206, stabilin-1) expression were analyzed by ELISA and confocal microscopy respectively. BGD-modified materials have improved thermal stability and good mechanical properties. However, BGD modifications induced additional donor-specific inflammatory reactions and suppressed tolerogenic phenotype of macrophages. Therefore, our test-system successfully demonstrated specific immunomodulatory effects of original and modified PLA-based biomaterials, and can be further applied for the examination of improved coatings for implants and identification of patient-specific reactions to implants.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalMaterials Science and Engineering C
Volume51
DOIs
Publication statusPublished - 1 Jun 2015

Fingerprint

monocytes
macrophages
Macrophages
Biocompatible Materials
Biomaterials
Surface treatment
therapy
acids
Acids
Coloring Agents
Dyes
dyes
microscopy
coatings
Coatings
secretions
phenotype
Confocal microscopy
Tensile strain
biomarkers

Keywords

  • Biocompatibility
  • Cytokines
  • Macrophages
  • Monocytes
  • Polylactic acid
  • Surface modification

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

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title = "Surface modification of biomaterials based on high-molecular polylactic acid and their effect on inflammatory reactions of primary human monocyte-derived macrophages: Perspective for personalized therapy",
abstract = "Polylactic acid (PLA) based implants can cause inflammatory complications. Macrophages are key innate immune cells that control inflammation. To provide higher biocompatibility of PLA-based implants with local innate immune cells their surface properties have to be improved. In our study surface modification technique for high-molecular PLA (MW = 1,646,600 g/mol) based biomaterials was originally developed and successfully applied. Optimal modification conditions were determined. Treatment of PLA films with toluene/ethanol = 3/7 mixture for 10 min with subsequent exposure in 0.001 M brilliant green dye (BGD) solution allows to entrap approximately 10- 9 mol/cm2 model biomolecules. The modified PLA film surface was characterized by optical microscopy, SERS, FT-IR, UV and TG/DTA/DSC analysis. Tensile strain of modified films was determined as well. The effect of PLA films modified with BGD on the inflammatory reactions of primary human monocyte-derived macrophages was investigated. We developed in vitro test-system by differentiating primary monocyte-derived macrophages on a coating material. Type 1 and type 2 inflammatory cytokines (TNFα, CCL18) secretion and histological biomarkers (CD206, stabilin-1) expression were analyzed by ELISA and confocal microscopy respectively. BGD-modified materials have improved thermal stability and good mechanical properties. However, BGD modifications induced additional donor-specific inflammatory reactions and suppressed tolerogenic phenotype of macrophages. Therefore, our test-system successfully demonstrated specific immunomodulatory effects of original and modified PLA-based biomaterials, and can be further applied for the examination of improved coatings for implants and identification of patient-specific reactions to implants.",
keywords = "Biocompatibility, Cytokines, Macrophages, Monocytes, Polylactic acid, Surface modification",
author = "Ksenia Sergeevna Stankevich and Alexandru Gudima and Filimonov, {Victor D.} and Harald Kl{\"u}ter and Mamontova, {Evgeniya M.} and Tverdokhlebov, {Sergei I.} and Julia Kzhyshkowska",
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T2 - Perspective for personalized therapy

AU - Stankevich, Ksenia Sergeevna

AU - Gudima, Alexandru

AU - Filimonov, Victor D.

AU - Klüter, Harald

AU - Mamontova, Evgeniya M.

AU - Tverdokhlebov, Sergei I.

AU - Kzhyshkowska, Julia

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Polylactic acid (PLA) based implants can cause inflammatory complications. Macrophages are key innate immune cells that control inflammation. To provide higher biocompatibility of PLA-based implants with local innate immune cells their surface properties have to be improved. In our study surface modification technique for high-molecular PLA (MW = 1,646,600 g/mol) based biomaterials was originally developed and successfully applied. Optimal modification conditions were determined. Treatment of PLA films with toluene/ethanol = 3/7 mixture for 10 min with subsequent exposure in 0.001 M brilliant green dye (BGD) solution allows to entrap approximately 10- 9 mol/cm2 model biomolecules. The modified PLA film surface was characterized by optical microscopy, SERS, FT-IR, UV and TG/DTA/DSC analysis. Tensile strain of modified films was determined as well. The effect of PLA films modified with BGD on the inflammatory reactions of primary human monocyte-derived macrophages was investigated. We developed in vitro test-system by differentiating primary monocyte-derived macrophages on a coating material. Type 1 and type 2 inflammatory cytokines (TNFα, CCL18) secretion and histological biomarkers (CD206, stabilin-1) expression were analyzed by ELISA and confocal microscopy respectively. BGD-modified materials have improved thermal stability and good mechanical properties. However, BGD modifications induced additional donor-specific inflammatory reactions and suppressed tolerogenic phenotype of macrophages. Therefore, our test-system successfully demonstrated specific immunomodulatory effects of original and modified PLA-based biomaterials, and can be further applied for the examination of improved coatings for implants and identification of patient-specific reactions to implants.

AB - Polylactic acid (PLA) based implants can cause inflammatory complications. Macrophages are key innate immune cells that control inflammation. To provide higher biocompatibility of PLA-based implants with local innate immune cells their surface properties have to be improved. In our study surface modification technique for high-molecular PLA (MW = 1,646,600 g/mol) based biomaterials was originally developed and successfully applied. Optimal modification conditions were determined. Treatment of PLA films with toluene/ethanol = 3/7 mixture for 10 min with subsequent exposure in 0.001 M brilliant green dye (BGD) solution allows to entrap approximately 10- 9 mol/cm2 model biomolecules. The modified PLA film surface was characterized by optical microscopy, SERS, FT-IR, UV and TG/DTA/DSC analysis. Tensile strain of modified films was determined as well. The effect of PLA films modified with BGD on the inflammatory reactions of primary human monocyte-derived macrophages was investigated. We developed in vitro test-system by differentiating primary monocyte-derived macrophages on a coating material. Type 1 and type 2 inflammatory cytokines (TNFα, CCL18) secretion and histological biomarkers (CD206, stabilin-1) expression were analyzed by ELISA and confocal microscopy respectively. BGD-modified materials have improved thermal stability and good mechanical properties. However, BGD modifications induced additional donor-specific inflammatory reactions and suppressed tolerogenic phenotype of macrophages. Therefore, our test-system successfully demonstrated specific immunomodulatory effects of original and modified PLA-based biomaterials, and can be further applied for the examination of improved coatings for implants and identification of patient-specific reactions to implants.

KW - Biocompatibility

KW - Cytokines

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KW - Monocytes

KW - Polylactic acid

KW - Surface modification

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