Superparamagnetic cobalt ferrite nanoparticles “blow up” the spatial ordering of double-stranded DNA molecules

Yu M. Yevdokimov, A. G. Pershina, V. I. Salyanov, A. A. Magaeva, V. I. Popenko, E. V. Shtykova, L. A. Dadinova, S. G. Skuridin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A study was made of the formation of cholesteric liquid-crystalline dispersions (ChLCDs) of double-stranded DNA molecules that were treated with positively charged superparamagnetic cobalt ferrite nanoparticles and the effect the particles exert on DNA liquid-crystalline dispersions (LCDs). When magnetic nanoparticles (MNPs) were bound to linear double-stranded DNA molecules at a high ionic strength (0.3 M NaCl), subsequent phase exclusion of the complexes from a polyethylene glycol (PEG)-containing solution failed to produce a dispersion wherein particles have a spatially twisted arrangement of neighbor double-stranded DNA molecules. When MNPs were added to a DNA ChCLD (1 MNP per 1 DNA molecule), the DNA structure was distorted at MNP binding sites so that the spatial ordering of DNA LCD particles was blown up, abolishing both abnormal optical activity and the characteristic Bragg maximum on a small-angle X-ray scattering (SAXS) curve. The effect may have important biological consequences, considering that physicochemical properties of double-stranded DNA LCD particles reflect features of the DNA spatial organization in chromosomes of primitive organisms.

Original languageEnglish
Article numberA004
Pages (from-to)341-347
Number of pages7
JournalBiophysics (Russian Federation)
Volume60
Issue number3
DOIs
Publication statusPublished - 1 May 2015
Externally publishedYes

Fingerprint

Nanoparticles
DNA
cobalt ferrite
Optical Rotation
Osmolar Concentration
Chromosomes
Binding Sites
X-Rays

Keywords

  • Circular dichroism
  • Liquid-crystalline dispersions
  • Small-angle X-ray scattering
  • Superparamagnetic cobalt ferrite nanoparticles

ASJC Scopus subject areas

  • Biophysics

Cite this

Yevdokimov, Y. M., Pershina, A. G., Salyanov, V. I., Magaeva, A. A., Popenko, V. I., Shtykova, E. V., ... Skuridin, S. G. (2015). Superparamagnetic cobalt ferrite nanoparticles “blow up” the spatial ordering of double-stranded DNA molecules. Biophysics (Russian Federation), 60(3), 341-347. [A004]. https://doi.org/10.1134/S0006350915030057

Superparamagnetic cobalt ferrite nanoparticles “blow up” the spatial ordering of double-stranded DNA molecules. / Yevdokimov, Yu M.; Pershina, A. G.; Salyanov, V. I.; Magaeva, A. A.; Popenko, V. I.; Shtykova, E. V.; Dadinova, L. A.; Skuridin, S. G.

In: Biophysics (Russian Federation), Vol. 60, No. 3, A004, 01.05.2015, p. 341-347.

Research output: Contribution to journalArticle

Yevdokimov, YM, Pershina, AG, Salyanov, VI, Magaeva, AA, Popenko, VI, Shtykova, EV, Dadinova, LA & Skuridin, SG 2015, 'Superparamagnetic cobalt ferrite nanoparticles “blow up” the spatial ordering of double-stranded DNA molecules', Biophysics (Russian Federation), vol. 60, no. 3, A004, pp. 341-347. https://doi.org/10.1134/S0006350915030057
Yevdokimov, Yu M. ; Pershina, A. G. ; Salyanov, V. I. ; Magaeva, A. A. ; Popenko, V. I. ; Shtykova, E. V. ; Dadinova, L. A. ; Skuridin, S. G. / Superparamagnetic cobalt ferrite nanoparticles “blow up” the spatial ordering of double-stranded DNA molecules. In: Biophysics (Russian Federation). 2015 ; Vol. 60, No. 3. pp. 341-347.
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