Abstract
A study was made of the formation of cholesteric liquid-crystalline dispersions (ChLCDs) of double-stranded DNA molecules that were treated with positively charged superparamagnetic cobalt ferrite nanoparticles and the effect the particles exert on DNA liquid-crystalline dispersions (LCDs). When magnetic nanoparticles (MNPs) were bound to linear double-stranded DNA molecules at a high ionic strength (0.3 M NaCl), subsequent phase exclusion of the complexes from a polyethylene glycol (PEG)-containing solution failed to produce a dispersion wherein particles have a spatially twisted arrangement of neighbor double-stranded DNA molecules. When MNPs were added to a DNA ChCLD (1 MNP per 1 DNA molecule), the DNA structure was distorted at MNP binding sites so that the spatial ordering of DNA LCD particles was blown up, abolishing both abnormal optical activity and the characteristic Bragg maximum on a small-angle X-ray scattering (SAXS) curve. The effect may have important biological consequences, considering that physicochemical properties of double-stranded DNA LCD particles reflect features of the DNA spatial organization in chromosomes of primitive organisms.
| Original language | English |
|---|---|
| Article number | A004 |
| Pages (from-to) | 341-347 |
| Number of pages | 7 |
| Journal | Biophysics (Russian Federation) |
| Volume | 60 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 May 2015 |
| Externally published | Yes |
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Keywords
- Circular dichroism
- Liquid-crystalline dispersions
- Small-angle X-ray scattering
- Superparamagnetic cobalt ferrite nanoparticles
ASJC Scopus subject areas
- Biophysics
Cite this
Superparamagnetic cobalt ferrite nanoparticles “blow up” the spatial ordering of double-stranded DNA molecules. / Yevdokimov, Yu M.; Pershina, A. G.; Salyanov, V. I.; Magaeva, A. A.; Popenko, V. I.; Shtykova, E. V.; Dadinova, L. A.; Skuridin, S. G.
In: Biophysics (Russian Federation), Vol. 60, No. 3, A004, 01.05.2015, p. 341-347.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Superparamagnetic cobalt ferrite nanoparticles “blow up” the spatial ordering of double-stranded DNA molecules
AU - Yevdokimov, Yu M.
AU - Pershina, A. G.
AU - Salyanov, V. I.
AU - Magaeva, A. A.
AU - Popenko, V. I.
AU - Shtykova, E. V.
AU - Dadinova, L. A.
AU - Skuridin, S. G.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - A study was made of the formation of cholesteric liquid-crystalline dispersions (ChLCDs) of double-stranded DNA molecules that were treated with positively charged superparamagnetic cobalt ferrite nanoparticles and the effect the particles exert on DNA liquid-crystalline dispersions (LCDs). When magnetic nanoparticles (MNPs) were bound to linear double-stranded DNA molecules at a high ionic strength (0.3 M NaCl), subsequent phase exclusion of the complexes from a polyethylene glycol (PEG)-containing solution failed to produce a dispersion wherein particles have a spatially twisted arrangement of neighbor double-stranded DNA molecules. When MNPs were added to a DNA ChCLD (1 MNP per 1 DNA molecule), the DNA structure was distorted at MNP binding sites so that the spatial ordering of DNA LCD particles was blown up, abolishing both abnormal optical activity and the characteristic Bragg maximum on a small-angle X-ray scattering (SAXS) curve. The effect may have important biological consequences, considering that physicochemical properties of double-stranded DNA LCD particles reflect features of the DNA spatial organization in chromosomes of primitive organisms.
AB - A study was made of the formation of cholesteric liquid-crystalline dispersions (ChLCDs) of double-stranded DNA molecules that were treated with positively charged superparamagnetic cobalt ferrite nanoparticles and the effect the particles exert on DNA liquid-crystalline dispersions (LCDs). When magnetic nanoparticles (MNPs) were bound to linear double-stranded DNA molecules at a high ionic strength (0.3 M NaCl), subsequent phase exclusion of the complexes from a polyethylene glycol (PEG)-containing solution failed to produce a dispersion wherein particles have a spatially twisted arrangement of neighbor double-stranded DNA molecules. When MNPs were added to a DNA ChCLD (1 MNP per 1 DNA molecule), the DNA structure was distorted at MNP binding sites so that the spatial ordering of DNA LCD particles was blown up, abolishing both abnormal optical activity and the characteristic Bragg maximum on a small-angle X-ray scattering (SAXS) curve. The effect may have important biological consequences, considering that physicochemical properties of double-stranded DNA LCD particles reflect features of the DNA spatial organization in chromosomes of primitive organisms.
KW - Circular dichroism
KW - Liquid-crystalline dispersions
KW - Small-angle X-ray scattering
KW - Superparamagnetic cobalt ferrite nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84943275930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84943275930&partnerID=8YFLogxK
U2 - 10.1134/S0006350915030057
DO - 10.1134/S0006350915030057
M3 - Article
AN - SCOPUS:84943275930
VL - 60
SP - 341
EP - 347
JO - Biophysics (Russian Federation)
JF - Biophysics (Russian Federation)
SN - 0006-3509
IS - 3
M1 - A004
ER -