Sulfidation of silver nanowires inside human alveolar epithelial cells

A potential detoxification mechanism

Shu Chen, Angela E. Goode, Sinbad Sweeney, Ioannis G. Theodorou, Andrew J. Thorley, Pakatip Ruenraroengsak, Yan Chang, Andrew Gow, Stephan Schwander, Jeremy Skepper, Junfeng Zhang, Milo S. Shaffer, Kian Fan Chung, Teresa D. Tetley, Mary P. Ryan, Alexandra E. Porter

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Silver nanowires (AgNWs) are being developed for use in optoelectronics. However before widespread usage, it is crucial to determine their potential effects on human health. It is accepted that Ag nanoparticles (AgNPs) exert toxic effects by releasing Ag+ ions, but much less is known about whether Ag+ reacts with compounds, or any downstream bioactive effects of transformed AgNPs. Analytical high-resolution transmission electron microscopy has been employed to elucidate cellular uptake and reactivity of AgNWs inside human alveolar epithelial type 1-like cells. AgNWs were observed in the cytoplasm and membrane-bound vesicles, and precipitation of Ag2S within the cell occurred after 1 h exposure. Cell viability studies showed no evidence of cytotoxicity and reactive oxygen species were not observed on exposure of cells to AgNWs. We suggest that Ag2S formation acts as a 'trap' for free Ag+, significantly limiting short-term toxicological effects-with important consequences for the safety of Ag-nanomaterials to human health.

Original languageEnglish
Pages (from-to)9839-9847
Number of pages9
JournalNanoscale
Volume5
Issue number20
DOIs
Publication statusPublished - 21 Oct 2013
Externally publishedYes

Fingerprint

Nanowires
Alveolar Epithelial Cells
Detoxification
Silver
Health
Nanoparticles
Poisons
Cytotoxicity
High resolution transmission electron microscopy
Nanostructured materials
Optoelectronic devices
Reactive Oxygen Species
Nanostructures
Cells
Ions
Membranes
Transmission Electron Microscopy
Toxicology
Oxygen
Cell Survival

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

Chen, S., Goode, A. E., Sweeney, S., Theodorou, I. G., Thorley, A. J., Ruenraroengsak, P., ... Porter, A. E. (2013). Sulfidation of silver nanowires inside human alveolar epithelial cells: A potential detoxification mechanism. Nanoscale, 5(20), 9839-9847. https://doi.org/10.1039/c3nr03205a

Sulfidation of silver nanowires inside human alveolar epithelial cells : A potential detoxification mechanism. / Chen, Shu; Goode, Angela E.; Sweeney, Sinbad; Theodorou, Ioannis G.; Thorley, Andrew J.; Ruenraroengsak, Pakatip; Chang, Yan; Gow, Andrew; Schwander, Stephan; Skepper, Jeremy; Zhang, Junfeng; Shaffer, Milo S.; Chung, Kian Fan; Tetley, Teresa D.; Ryan, Mary P.; Porter, Alexandra E.

In: Nanoscale, Vol. 5, No. 20, 21.10.2013, p. 9839-9847.

Research output: Contribution to journalArticle

Chen, S, Goode, AE, Sweeney, S, Theodorou, IG, Thorley, AJ, Ruenraroengsak, P, Chang, Y, Gow, A, Schwander, S, Skepper, J, Zhang, J, Shaffer, MS, Chung, KF, Tetley, TD, Ryan, MP & Porter, AE 2013, 'Sulfidation of silver nanowires inside human alveolar epithelial cells: A potential detoxification mechanism', Nanoscale, vol. 5, no. 20, pp. 9839-9847. https://doi.org/10.1039/c3nr03205a
Chen S, Goode AE, Sweeney S, Theodorou IG, Thorley AJ, Ruenraroengsak P et al. Sulfidation of silver nanowires inside human alveolar epithelial cells: A potential detoxification mechanism. Nanoscale. 2013 Oct 21;5(20):9839-9847. https://doi.org/10.1039/c3nr03205a
Chen, Shu ; Goode, Angela E. ; Sweeney, Sinbad ; Theodorou, Ioannis G. ; Thorley, Andrew J. ; Ruenraroengsak, Pakatip ; Chang, Yan ; Gow, Andrew ; Schwander, Stephan ; Skepper, Jeremy ; Zhang, Junfeng ; Shaffer, Milo S. ; Chung, Kian Fan ; Tetley, Teresa D. ; Ryan, Mary P. ; Porter, Alexandra E. / Sulfidation of silver nanowires inside human alveolar epithelial cells : A potential detoxification mechanism. In: Nanoscale. 2013 ; Vol. 5, No. 20. pp. 9839-9847.
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