Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays

Alla N. Generalova, Svetlana V. Sizova, Tatiana A. Zdobnova, Margarita M. Zarifullina, Michail V. Artemyev, Alexander V. Baranov, Vladimir A. Oleinikov, Vitaly P. Zubov, Sergey M. Deyev

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Aim: This study aimed to design a panel of uniform particulate biochemical reagents and to test them in specific bioassays. These reagents are polymer particles of different sizes doped with semiconductor nanocrystals and conjugated with either full-size antibodies or recombinant mini-antibodies (4D5 scFv fragment) designed by genetic engineering approaches. Materials & methods: A panel of highly fluorescent polymer particles (150-800 nm) were formed by embedding CdSe/ZnS nanocrystals (quantum dots) into preformed polyacrolein and poly(acrolein-co-styrene) particles. Morphology, content and fluorescence characteristics of the prepared materials were studied by laser correlation spectroscopy, spectrophotometry, optical and fluorescent microscopy and fluorimetry. Results: The obtained fluorescent particles sensitized by anti-Yersinia pestis antibodies were used for rapid agglutination glass test suitable for screening analysis of Y. pestis antigen and for microtiter particle agglutination, which, owing to its speed and simplicity, is very beneficial for diagnostic detection of Y. pestis antigen. Recombinant 4D5 scFv antibodies designed and conjugated with polymer particles containing quantum dots provide multipoint highly specific binding with cancer marker HER2/neu on the surface of SKOV-3 cell.

Original languageEnglish
Pages (from-to)195-209
Number of pages15
JournalNanomedicine
Volume6
Issue number2
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Keywords

  • antibody
  • cancer cell
  • cell imaging
  • HER2/neu
  • polymer particle
  • polymer particle agglutination
  • quantum dot
  • recombinant antibody
  • synthesis
  • Yersinia pestis antigen

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering
  • Development
  • Materials Science(all)

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  • Cite this

    Generalova, A. N., Sizova, S. V., Zdobnova, T. A., Zarifullina, M. M., Artemyev, M. V., Baranov, A. V., Oleinikov, V. A., Zubov, V. P., & Deyev, S. M. (2011). Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays. Nanomedicine, 6(2), 195-209. https://doi.org/10.2217/nnm.10.162