Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays

Alla N. Generalova, Svetlana V. Sizova, Tatiana A. Zdobnova, Margarita M. Zarifullina, Michail V. Artemyev, Alexander V. Baranov, Vladimir A. Oleinikov, Vitaly P. Zubov, Sergey M. Deyev

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Aim: This study aimed to design a panel of uniform particulate biochemical reagents and to test them in specific bioassays. These reagents are polymer particles of different sizes doped with semiconductor nanocrystals and conjugated with either full-size antibodies or recombinant mini-antibodies (4D5 scFv fragment) designed by genetic engineering approaches. Materials & methods: A panel of highly fluorescent polymer particles (150-800 nm) were formed by embedding CdSe/ZnS nanocrystals (quantum dots) into preformed polyacrolein and poly(acrolein-co-styrene) particles. Morphology, content and fluorescence characteristics of the prepared materials were studied by laser correlation spectroscopy, spectrophotometry, optical and fluorescent microscopy and fluorimetry. Results: The obtained fluorescent particles sensitized by anti-Yersinia pestis antibodies were used for rapid agglutination glass test suitable for screening analysis of Y. pestis antigen and for microtiter particle agglutination, which, owing to its speed and simplicity, is very beneficial for diagnostic detection of Y. pestis antigen. Recombinant 4D5 scFv antibodies designed and conjugated with polymer particles containing quantum dots provide multipoint highly specific binding with cancer marker HER2/neu on the surface of SKOV-3 cell.

Original languageEnglish
Pages (from-to)195-209
Number of pages15
JournalNanomedicine
Volume6
Issue number2
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Fingerprint

Yersinia pestis
Quantum Dots
Bioassay
Antibodies
Biological Assay
Nanocrystals
bioassay
Single-Chain Antibodies
Polymers
polymer
Semiconductor materials
genetic engineering
Semiconductor quantum dots
antibody
Antigens
Acrolein
Genetic engineering
Fluorometry
Agglutination Tests
diagnostic

Keywords

  • antibody
  • cancer cell
  • cell imaging
  • HER2/neu
  • polymer particle
  • polymer particle agglutination
  • quantum dot
  • recombinant antibody
  • synthesis
  • Yersinia pestis antigen

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering
  • Development
  • Materials Science(all)

Cite this

Generalova, A. N., Sizova, S. V., Zdobnova, T. A., Zarifullina, M. M., Artemyev, M. V., Baranov, A. V., ... Deyev, S. M. (2011). Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays. Nanomedicine, 6(2), 195-209. https://doi.org/10.2217/nnm.10.162

Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays. / Generalova, Alla N.; Sizova, Svetlana V.; Zdobnova, Tatiana A.; Zarifullina, Margarita M.; Artemyev, Michail V.; Baranov, Alexander V.; Oleinikov, Vladimir A.; Zubov, Vitaly P.; Deyev, Sergey M.

In: Nanomedicine, Vol. 6, No. 2, 02.2011, p. 195-209.

Research output: Contribution to journalArticle

Generalova, AN, Sizova, SV, Zdobnova, TA, Zarifullina, MM, Artemyev, MV, Baranov, AV, Oleinikov, VA, Zubov, VP & Deyev, SM 2011, 'Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays', Nanomedicine, vol. 6, no. 2, pp. 195-209. https://doi.org/10.2217/nnm.10.162
Generalova AN, Sizova SV, Zdobnova TA, Zarifullina MM, Artemyev MV, Baranov AV et al. Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays. Nanomedicine. 2011 Feb;6(2):195-209. https://doi.org/10.2217/nnm.10.162
Generalova, Alla N. ; Sizova, Svetlana V. ; Zdobnova, Tatiana A. ; Zarifullina, Margarita M. ; Artemyev, Michail V. ; Baranov, Alexander V. ; Oleinikov, Vladimir A. ; Zubov, Vitaly P. ; Deyev, Sergey M. / Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays. In: Nanomedicine. 2011 ; Vol. 6, No. 2. pp. 195-209.
@article{142d5ebb814540848671962cb698fe5e,
title = "Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays",
abstract = "Aim: This study aimed to design a panel of uniform particulate biochemical reagents and to test them in specific bioassays. These reagents are polymer particles of different sizes doped with semiconductor nanocrystals and conjugated with either full-size antibodies or recombinant mini-antibodies (4D5 scFv fragment) designed by genetic engineering approaches. Materials & methods: A panel of highly fluorescent polymer particles (150-800 nm) were formed by embedding CdSe/ZnS nanocrystals (quantum dots) into preformed polyacrolein and poly(acrolein-co-styrene) particles. Morphology, content and fluorescence characteristics of the prepared materials were studied by laser correlation spectroscopy, spectrophotometry, optical and fluorescent microscopy and fluorimetry. Results: The obtained fluorescent particles sensitized by anti-Yersinia pestis antibodies were used for rapid agglutination glass test suitable for screening analysis of Y. pestis antigen and for microtiter particle agglutination, which, owing to its speed and simplicity, is very beneficial for diagnostic detection of Y. pestis antigen. Recombinant 4D5 scFv antibodies designed and conjugated with polymer particles containing quantum dots provide multipoint highly specific binding with cancer marker HER2/neu on the surface of SKOV-3 cell.",
keywords = "antibody, cancer cell, cell imaging, HER2/neu, polymer particle, polymer particle agglutination, quantum dot, recombinant antibody, synthesis, Yersinia pestis antigen",
author = "Generalova, {Alla N.} and Sizova, {Svetlana V.} and Zdobnova, {Tatiana A.} and Zarifullina, {Margarita M.} and Artemyev, {Michail V.} and Baranov, {Alexander V.} and Oleinikov, {Vladimir A.} and Zubov, {Vitaly P.} and Deyev, {Sergey M.}",
year = "2011",
month = "2",
doi = "10.2217/nnm.10.162",
language = "English",
volume = "6",
pages = "195--209",
journal = "Nanomedicine",
issn = "1743-5889",
publisher = "Future Medicine Ltd.",
number = "2",

}

TY - JOUR

T1 - Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays

AU - Generalova, Alla N.

AU - Sizova, Svetlana V.

AU - Zdobnova, Tatiana A.

AU - Zarifullina, Margarita M.

AU - Artemyev, Michail V.

AU - Baranov, Alexander V.

AU - Oleinikov, Vladimir A.

AU - Zubov, Vitaly P.

AU - Deyev, Sergey M.

PY - 2011/2

Y1 - 2011/2

N2 - Aim: This study aimed to design a panel of uniform particulate biochemical reagents and to test them in specific bioassays. These reagents are polymer particles of different sizes doped with semiconductor nanocrystals and conjugated with either full-size antibodies or recombinant mini-antibodies (4D5 scFv fragment) designed by genetic engineering approaches. Materials & methods: A panel of highly fluorescent polymer particles (150-800 nm) were formed by embedding CdSe/ZnS nanocrystals (quantum dots) into preformed polyacrolein and poly(acrolein-co-styrene) particles. Morphology, content and fluorescence characteristics of the prepared materials were studied by laser correlation spectroscopy, spectrophotometry, optical and fluorescent microscopy and fluorimetry. Results: The obtained fluorescent particles sensitized by anti-Yersinia pestis antibodies were used for rapid agglutination glass test suitable for screening analysis of Y. pestis antigen and for microtiter particle agglutination, which, owing to its speed and simplicity, is very beneficial for diagnostic detection of Y. pestis antigen. Recombinant 4D5 scFv antibodies designed and conjugated with polymer particles containing quantum dots provide multipoint highly specific binding with cancer marker HER2/neu on the surface of SKOV-3 cell.

AB - Aim: This study aimed to design a panel of uniform particulate biochemical reagents and to test them in specific bioassays. These reagents are polymer particles of different sizes doped with semiconductor nanocrystals and conjugated with either full-size antibodies or recombinant mini-antibodies (4D5 scFv fragment) designed by genetic engineering approaches. Materials & methods: A panel of highly fluorescent polymer particles (150-800 nm) were formed by embedding CdSe/ZnS nanocrystals (quantum dots) into preformed polyacrolein and poly(acrolein-co-styrene) particles. Morphology, content and fluorescence characteristics of the prepared materials were studied by laser correlation spectroscopy, spectrophotometry, optical and fluorescent microscopy and fluorimetry. Results: The obtained fluorescent particles sensitized by anti-Yersinia pestis antibodies were used for rapid agglutination glass test suitable for screening analysis of Y. pestis antigen and for microtiter particle agglutination, which, owing to its speed and simplicity, is very beneficial for diagnostic detection of Y. pestis antigen. Recombinant 4D5 scFv antibodies designed and conjugated with polymer particles containing quantum dots provide multipoint highly specific binding with cancer marker HER2/neu on the surface of SKOV-3 cell.

KW - antibody

KW - cancer cell

KW - cell imaging

KW - HER2/neu

KW - polymer particle

KW - polymer particle agglutination

KW - quantum dot

KW - recombinant antibody

KW - synthesis

KW - Yersinia pestis antigen

UR - http://www.scopus.com/inward/record.url?scp=79952606427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952606427&partnerID=8YFLogxK

U2 - 10.2217/nnm.10.162

DO - 10.2217/nnm.10.162

M3 - Article

VL - 6

SP - 195

EP - 209

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 2

ER -