Studying cGMP-dependent mechanisms of vinpocetine on smooth muscle cells

I. V. Kovalev, M. B. Baskakov, A. G. Popov, A. A. Kilin, I. L. Minochenko, Yu L. Borodin, A. A. Panov, Yana Jonovna Anfinogenova, L. V. Kapilevich, M. A. Medvedev

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Abstract

The results of the membrane potential measurements (by the double sucrose gap junction technique) and the smooth muscle tension determination (by the mechanical force measurements) in the rat aorta showed that vinpocetine potentiates the effect of sodium nitroprusside and nitroglycerin on the smooth muscle cells. In the concentration range of 2-20 μM, vinpocetine produced a dose-dependent inhibition of the Ca2+ conductivity of the membrane and decreased the smooth muscle contractility response. At a concentration of 1 μM, the drug acted as an inhibitor of the phosphodiestherase (PDE) activity and produced the effects similar top those of dibutyryl-cGMP (rather than dibutyryl-cAMP). In the presence of 10 μM of Methylene Blue (an inhibitor of the soluble fraction of guanylate cyclase), the cGMP-dependent effects of vinpocetine were suppressed on the background of 100 μM of sodium nitroprusside, but retained on the background of 10 μM of 3-isobutyl-1-methylxanthine (IBMX), a nonspecific PDE inhibitor. IBMX acted like dibityryl-cGMP and activated the K+ conductivity of the membrane. It is suggested that cGMP-dependent effects of vinpocetine are related to its action upon the Ca2+ and Na+ (but not K+) conductivity and to the cGMP-induced increase in the contribution of sarcoplasmic calcium to the contractile response.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalEksperimental'naya i Klinicheskaya Farmakologiya
Volume66
Issue number4
Publication statusPublished - 2003
Externally publishedYes

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ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Kovalev, I. V., Baskakov, M. B., Popov, A. G., Kilin, A. A., Minochenko, I. L., Borodin, Y. L., Panov, A. A., Anfinogenova, Y. J., Kapilevich, L. V., & Medvedev, M. A. (2003). Studying cGMP-dependent mechanisms of vinpocetine on smooth muscle cells. Eksperimental'naya i Klinicheskaya Farmakologiya, 66(4), 25-28.