Structural and Functional Characterization of the rpb8+ Gene of Schizosaccharomyces pombe Encoding a Subunit of RNA Polymerases I-III only Specific for Eukaryotes

G. V. Shpakovski, S. A. Proshkin, A. L. Kayushin, M. D. Korosteleva, E. N. Lebedenko

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1 Citation (Scopus)

Abstract

A full-length cDNA of the rpb8+ gene encoding a common subunit Rpb8 of nuclear RNA polymerases I-III only specific for Eucarya was isolated from an expression library of the fission yeast Schizosaccharomyces pombe. The primary structure of the corresponding fragment of the Sz. pombe genome was also established. The rpb8+ gene contains two short introns, 59 and 48 bp long. Only short segments of homology were found upon comparing the Rpb8 subunit homologs from various eukaryotic species, and substantial differencies exist between the corresponding proteins of unicellular and multicellular organisms. Subunit Rpb8 of Sz. pombe proved to be the smallest one among the known related proteins: it lacks the 21-aa fragment corresponding to amino acids residues 68-88 of the central part of the homologous subunit ABC14.5 of Saccharomyces cerevisiae. Accordingly, subunit Rpb8 of the fission yeast was not capable of substituting in vivo subunit ABC14.5 in nuclear RNA polymerases of the baker's yeast.

Original languageEnglish
Pages (from-to)125
Number of pages1
JournalBioorganicheskaya Khimiya
Volume24
Issue number2
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • Common subunit Rpb8
  • Fission yeast
  • Introns
  • Nuclear RNA polymerases I-III
  • rpb8 gene

ASJC Scopus subject areas

  • Medicine(all)

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