Structural and functional alterations in the atrioventricular node and atrioventricular ring tissue in ischaemia-induced heart failure

Joseph Yanni, Michal Maczewski, Urszula Mackiewicz, Samuel Siew, Olga Fedorenko, Andrew Atkinson, Marcus Price, Andrzej Beresewicz, Robert H. Anderson, Mark R. Boyett, Halina Dobrzynski

Research output: Contribution to journalArticle

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Abstract

Heart failure (HF) causes dysfunction of the atrioventricular node (AVN) - first or second-degree heart block is a risk factor for sudden cardiac death in HF patients. The aim of the study was to determine if HF causes remodelling of the AVN and right atrioventricular ring (RAVR). HF was induced in rats (n=4) by ligation of the proximal left coronary artery, which resulted in a large infarct of the left ventricle. Sham-operated rats (n=4) were used as controls. Eight weeks after surgery, functional experiments were performed and the hearts were frozen. The body weight of HF rats was similar to control rats, but the mean heart weight of HF rats was significantly enlarged. In HF rats compared to controls, the left ventricle was dilated, left ventricular enddiastolic pressure elevated (21.0±0.6 and 5.4±0.2 mm Hg), left ventricular ejection fraction reduced (0.2±0.02 and 0.5±0.02) and left ventricular end-systolic pressure reduced (102±4.2 and 127±3.1 mm Hg). In HF rats, the in vivo and in vitro PR intervals were increased (41% and 20%), as was the Wenckebach cycle length, indicative of AVN dysfunction. The collagen content was significantly increased in the AVN and RAVR indicating fibrosis. Immunolabelling of caveolin3 (cell membrane marker) showed that there was hypertrophy in HF (cell diameter was increased by 63%, 39% in AVN, RAVR). The TUNEL assay showed that the myocytes of the AVN and RAVR in HF undergo apoptotic cell death. Immunolabelling showed that expression of HCN4 was significantly decreased in the AVN and RAVR (43% and 47%) in HF. We conclude that in HF there is remodelling of the AVN and RAVR and this remodelling may explain the AVN dysfunction.

Original languageEnglish
Pages (from-to)891-902
Number of pages12
JournalHistology and Histopathology
Volume29
Issue number7
Publication statusPublished - 2014

Fingerprint

Atrioventricular Node
Ischemia
Heart Failure
Heart Ventricles
Heart Block
Sudden Cardiac Death
In Situ Nick-End Labeling
Ventricular Pressure
Stroke Volume
Muscle Cells
Hypertrophy
Ligation
Coronary Vessels
Fibrosis
Cell Death
Collagen

Keywords

  • Atrioventricular node
  • Atrioventricular ring
  • Heart failure

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Structural and functional alterations in the atrioventricular node and atrioventricular ring tissue in ischaemia-induced heart failure. / Yanni, Joseph; Maczewski, Michal; Mackiewicz, Urszula; Siew, Samuel; Fedorenko, Olga; Atkinson, Andrew; Price, Marcus; Beresewicz, Andrzej; Anderson, Robert H.; Boyett, Mark R.; Dobrzynski, Halina.

In: Histology and Histopathology, Vol. 29, No. 7, 2014, p. 891-902.

Research output: Contribution to journalArticle

Yanni, J, Maczewski, M, Mackiewicz, U, Siew, S, Fedorenko, O, Atkinson, A, Price, M, Beresewicz, A, Anderson, RH, Boyett, MR & Dobrzynski, H 2014, 'Structural and functional alterations in the atrioventricular node and atrioventricular ring tissue in ischaemia-induced heart failure', Histology and Histopathology, vol. 29, no. 7, pp. 891-902.
Yanni, Joseph ; Maczewski, Michal ; Mackiewicz, Urszula ; Siew, Samuel ; Fedorenko, Olga ; Atkinson, Andrew ; Price, Marcus ; Beresewicz, Andrzej ; Anderson, Robert H. ; Boyett, Mark R. ; Dobrzynski, Halina. / Structural and functional alterations in the atrioventricular node and atrioventricular ring tissue in ischaemia-induced heart failure. In: Histology and Histopathology. 2014 ; Vol. 29, No. 7. pp. 891-902.
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