Spheroids of her2-positive breast adenocarcinoma for studying anticancer immunotoxins in vitro

I. V. Balalaeva, E. A. Sokolova, A. D. Puzhikhina, A. A. Brilkina, S. M. Deyev

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Tumor response to therapeutic treatment is largely determined by its heterogeneity and the presence of intercellular junctions, hindering the penetration of large molecules deep into the three-dimensional structure of the tumor. In that context, 3D in vitro tumor models such as cancer cell spheroids are becoming increasingly popular. We obtained spheroids of human breast adenocarcinoma SKBR-3 overexpressing the HER2 cancer marker. The toxicity of HER2-targeted immunotoxin 4D5scFv-PE40 against spheroids was shown to be several orders of magnitude lower compared to a monolayer cell culture. The significant difference in the severity of the immunotoxin effect can be explained by the fact that it ineffectively penetrates the spheroid and predominantly influences the cells of the outer layers. The resulting tumor spheroid model can be used in development of drugs for targeted therapy as well as to study ways to improve the efficiency of anticancer agents by targeting cell-cell contacts. 2017 Park-media, Ltd.

Original languageEnglish
Pages (from-to)38-43
Number of pages6
JournalActa Naturae
Volume9
Issue number1
Publication statusPublished - 2017

Keywords

  • 4D5scFv-PE40 immunotoxin
  • Cancer marker HER2
  • Drug penetration into tumor
  • Spheroids
  • Targeted therapy

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

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