Specific visualization of tumor cells using upconversion nanophosphors

E. A. Grebenik, A. N. Generalova, A. V. Nechaev, E. V. Khaydukov, K. E. Mironova, O. A. Stremovskiy, E. N. Lebedenko, A. V. Zvyagin, S. M. Deyev

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The development of targeted constructs on the basis of photoluminescent nanoparticles with a high photo- and chemical stability and absorption/emission spectra in the "transparency window" of biological tissues is an important focus area of present-day medical diagnostics. In this work, a targeted two-component construct on the basis of upconversion nanophosphors (UCNPs) and anti-tumor 4D5 scFv was developed for selective labeling of tumor cells overexpressing the HER2 tumor marker characteristic of a number of human malignant tumors. A high affinity barnase: barstar (Bn: Bs) protein pair, which exhibits high stability in a wide range of pH and temperatures, was exploited as a molecular adapter providing self-assembly of the two-component construct. High selectivity for the binding of the two-component 4D5 scFv-Bn: UCNP-Bs construct to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2 was demonstrated. This approach provides an opportunity to produce similar constructs for the visualization of different specific markers in pathogenic tissues, including malignant tumors.

Original languageEnglish
Pages (from-to)48-53
Number of pages6
JournalActa Naturae
Volume6
Issue number23
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Anti-tumor antibodies
  • Biomarker imaging
  • HER2
  • Self-assembly
  • Upconversion nanophosphors

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

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  • Cite this

    Grebenik, E. A., Generalova, A. N., Nechaev, A. V., Khaydukov, E. V., Mironova, K. E., Stremovskiy, O. A., Lebedenko, E. N., Zvyagin, A. V., & Deyev, S. M. (2014). Specific visualization of tumor cells using upconversion nanophosphors. Acta Naturae, 6(23), 48-53.