SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages

E. N. Atochina-Vasserman, E. V. Abramova, Y. Tomer, P. Scott, V. A. Nazarov, S. V. Kruglov, M. F. Beers, A. J. Gow, Igor Yu Malyshev

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This work was designed to study the role of surfactant protein D in the regulation of NO synthesis by "non-alveolar" microphages. We evaluated whether the effects of surfactant protein D depend on the phenotype of macrophages. In the absence of surfactant protein D, the LPS-induced iNOS response was shown to decrease in macrophages of native and proinflammatory phenotypes by 30%, and in macrophages of the antiinflammatory phenotype (by 63%). Under the influence of lipopolysaccharide in high doses (500 ng/ml), NO2 production by mouse macrophages without surfactant protein D was reduced in native cells (by 25%), but increased in proinflammatory (by 40%) and antiinflammatory phenotypes (by 12% compared to mouse macrophages with surfactant protein D). Our results suggest that surfactant protein D is involved in the immune response in the whole organism, but not only in the lungs. The effect of surfactant protein D depends on the phenotype of macrophages.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalBulletin of Experimental Biology and Medicine
Volume147
Issue number4
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Lipopolysaccharides
  • Macrophages
  • Nitric oxide
  • Surfactant protein D

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Atochina-Vasserman, E. N., Abramova, E. V., Tomer, Y., Scott, P., Nazarov, V. A., Kruglov, S. V., Beers, M. F., Gow, A. J., & Malyshev, I. Y. (2009). SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages. Bulletin of Experimental Biology and Medicine, 147(4), 415-420. https://doi.org/10.1007/s10517-009-0525-z