SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages

E. N. Atochina-Vasserman, E. V. Abramova, Y. Tomer, P. Scott, V. A. Nazarov, S. V. Kruglov, M. F. Beers, A. J. Gow, Igor Yu Malyshev

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This work was designed to study the role of surfactant protein D in the regulation of NO synthesis by "non-alveolar" microphages. We evaluated whether the effects of surfactant protein D depend on the phenotype of macrophages. In the absence of surfactant protein D, the LPS-induced iNOS response was shown to decrease in macrophages of native and proinflammatory phenotypes by 30%, and in macrophages of the antiinflammatory phenotype (by 63%). Under the influence of lipopolysaccharide in high doses (500 ng/ml), NO2 production by mouse macrophages without surfactant protein D was reduced in native cells (by 25%), but increased in proinflammatory (by 40%) and antiinflammatory phenotypes (by 12% compared to mouse macrophages with surfactant protein D). Our results suggest that surfactant protein D is involved in the immune response in the whole organism, but not only in the lungs. The effect of surfactant protein D depends on the phenotype of macrophages.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalBulletin of Experimental Biology and Medicine
Volume147
Issue number4
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein D
Macrophages
Peritoneal Macrophages
Metabolism
Lipopolysaccharides
Phenotype
Anti-Inflammatory Agents
Lung

Keywords

  • Lipopolysaccharides
  • Macrophages
  • Nitric oxide
  • Surfactant protein D

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages. / Atochina-Vasserman, E. N.; Abramova, E. V.; Tomer, Y.; Scott, P.; Nazarov, V. A.; Kruglov, S. V.; Beers, M. F.; Gow, A. J.; Malyshev, Igor Yu.

In: Bulletin of Experimental Biology and Medicine, Vol. 147, No. 4, 2009, p. 415-420.

Research output: Contribution to journalArticle

Atochina-Vasserman, EN, Abramova, EV, Tomer, Y, Scott, P, Nazarov, VA, Kruglov, SV, Beers, MF, Gow, AJ & Malyshev, IY 2009, 'SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages', Bulletin of Experimental Biology and Medicine, vol. 147, no. 4, pp. 415-420. https://doi.org/10.1007/s10517-009-0525-z
Atochina-Vasserman, E. N. ; Abramova, E. V. ; Tomer, Y. ; Scott, P. ; Nazarov, V. A. ; Kruglov, S. V. ; Beers, M. F. ; Gow, A. J. ; Malyshev, Igor Yu. / SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages. In: Bulletin of Experimental Biology and Medicine. 2009 ; Vol. 147, No. 4. pp. 415-420.
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